Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simpl...

Descripción completa

Detalles Bibliográficos
Autores principales: Crawford, Katharine H. D., Eguia, Rachel, Dingens, Adam S., Loes, Andrea N., Malone, Keara D., Wolf, Caitlin R., Chu, Helen Y., Tortorici, M. Alejandra, Veesler, David, Murphy, Michael, Pettie, Deleah, King, Neil P., Balazs, Alejandro B., Bloom, Jesse D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291041/
https://www.ncbi.nlm.nih.gov/pubmed/32384820
http://dx.doi.org/10.3390/v12050513

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291041/
https://www.ncbi.nlm.nih.gov/pubmed/32384820
http://dx.doi.org/10.3390/v12050513

Ejemplares similares