Cargando…
Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells
BACKGROUND: Astrocytes have been implicated in the pathophysiology of mood disorders and in the mechanism of the pharmacological effects of antidepressant drugs by the production of neurotrophic/growth factors. Previous studies have identified astrocyte‐expressed Gα(i/o)‐coupled lysophosphatidic aci...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292280/ https://www.ncbi.nlm.nih.gov/pubmed/31025529 http://dx.doi.org/10.1002/npr2.12055 |
_version_ | 1783546078715445248 |
---|---|
author | Abe, Hiromi Kajitani, Naoto Okada‐Tsuchioka, Mami Omori, Wataru Yatsumoto, Masahide Takebayashi, Minoru |
author_facet | Abe, Hiromi Kajitani, Naoto Okada‐Tsuchioka, Mami Omori, Wataru Yatsumoto, Masahide Takebayashi, Minoru |
author_sort | Abe, Hiromi |
collection | PubMed |
description | BACKGROUND: Astrocytes have been implicated in the pathophysiology of mood disorders and in the mechanism of the pharmacological effects of antidepressant drugs by the production of neurotrophic/growth factors. Previous studies have identified astrocyte‐expressed Gα(i/o)‐coupled lysophosphatidic acid receptor 1 (LPAR1), as being involved in antidepressant‐induced production of glial cell line‐derived neurotrophic factor (GDNF) and matrix metalloproteinase‐9 (MMP‐9) activation, an important step in the production of GNDF. However, the precise mechanism of MMP‐9 activation by antidepressants has yet to be identified, in particular the intracellular signaling pathway between LPAR1/Gα(i/o) and MMP‐9. METHODS AND RESULTS: Treatment of rat C6 astroglial cells (C6 cells) with amitriptyline increased Src family tyrosine kinase phosphorylation in a time and concentration‐dependent manner. Amitriptyline‐induced GDNF mRNA expression was blocked by Src family tyrosine kinase inhibitors. In addition, inhibiting Src family tyrosine kinase blocked amitriptyline‐induced zymographic MMP‐9 activation in C6 cells. The amitriptyline‐induced zymographic MMP‐9 activity was completely blocked by selective inhibition of Gα(i/o) protein and LPAR1. Furthermore, the amitriptyline‐induced Src family tyrosine kinase phosphorylation was blocked by LPAR1, but not MMP‐9 inhibition, indicating that Src family tyrosine kinase involvement is downstream of LPAR1. CONCLUSIONS: The current findings suggest that the pharmacological effect of antidepressant such as amitriptyline is mediated through an intracellular signaling pathway via the LPAR1/Gα(i/o)/Src family tyrosine kinase, which leads to MMP‐9 activation and GDNF production. |
format | Online Article Text |
id | pubmed-7292280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72922802020-12-08 Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells Abe, Hiromi Kajitani, Naoto Okada‐Tsuchioka, Mami Omori, Wataru Yatsumoto, Masahide Takebayashi, Minoru Neuropsychopharmacol Rep Original Articles BACKGROUND: Astrocytes have been implicated in the pathophysiology of mood disorders and in the mechanism of the pharmacological effects of antidepressant drugs by the production of neurotrophic/growth factors. Previous studies have identified astrocyte‐expressed Gα(i/o)‐coupled lysophosphatidic acid receptor 1 (LPAR1), as being involved in antidepressant‐induced production of glial cell line‐derived neurotrophic factor (GDNF) and matrix metalloproteinase‐9 (MMP‐9) activation, an important step in the production of GNDF. However, the precise mechanism of MMP‐9 activation by antidepressants has yet to be identified, in particular the intracellular signaling pathway between LPAR1/Gα(i/o) and MMP‐9. METHODS AND RESULTS: Treatment of rat C6 astroglial cells (C6 cells) with amitriptyline increased Src family tyrosine kinase phosphorylation in a time and concentration‐dependent manner. Amitriptyline‐induced GDNF mRNA expression was blocked by Src family tyrosine kinase inhibitors. In addition, inhibiting Src family tyrosine kinase blocked amitriptyline‐induced zymographic MMP‐9 activation in C6 cells. The amitriptyline‐induced zymographic MMP‐9 activity was completely blocked by selective inhibition of Gα(i/o) protein and LPAR1. Furthermore, the amitriptyline‐induced Src family tyrosine kinase phosphorylation was blocked by LPAR1, but not MMP‐9 inhibition, indicating that Src family tyrosine kinase involvement is downstream of LPAR1. CONCLUSIONS: The current findings suggest that the pharmacological effect of antidepressant such as amitriptyline is mediated through an intracellular signaling pathway via the LPAR1/Gα(i/o)/Src family tyrosine kinase, which leads to MMP‐9 activation and GDNF production. John Wiley and Sons Inc. 2019-04-25 /pmc/articles/PMC7292280/ /pubmed/31025529 http://dx.doi.org/10.1002/npr2.12055 Text en © 2019 The Authors. Neuropsychopharmacology Reports published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Neuropsychopharmacology This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Abe, Hiromi Kajitani, Naoto Okada‐Tsuchioka, Mami Omori, Wataru Yatsumoto, Masahide Takebayashi, Minoru Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells |
title | Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells |
title_full | Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells |
title_fullStr | Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells |
title_full_unstemmed | Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells |
title_short | Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells |
title_sort | antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mrna expression in rat astroglial cells |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292280/ https://www.ncbi.nlm.nih.gov/pubmed/31025529 http://dx.doi.org/10.1002/npr2.12055 |
work_keys_str_mv | AT abehiromi antidepressantamitriptylineinducedmatrixmetalloproteinase9activationismediatedbysrcfamilytyrosinekinasewhichleadstoglialcelllinederivedneurotrophicfactormrnaexpressioninratastroglialcells AT kajitaninaoto antidepressantamitriptylineinducedmatrixmetalloproteinase9activationismediatedbysrcfamilytyrosinekinasewhichleadstoglialcelllinederivedneurotrophicfactormrnaexpressioninratastroglialcells AT okadatsuchiokamami antidepressantamitriptylineinducedmatrixmetalloproteinase9activationismediatedbysrcfamilytyrosinekinasewhichleadstoglialcelllinederivedneurotrophicfactormrnaexpressioninratastroglialcells AT omoriwataru antidepressantamitriptylineinducedmatrixmetalloproteinase9activationismediatedbysrcfamilytyrosinekinasewhichleadstoglialcelllinederivedneurotrophicfactormrnaexpressioninratastroglialcells AT yatsumotomasahide antidepressantamitriptylineinducedmatrixmetalloproteinase9activationismediatedbysrcfamilytyrosinekinasewhichleadstoglialcelllinederivedneurotrophicfactormrnaexpressioninratastroglialcells AT takebayashiminoru antidepressantamitriptylineinducedmatrixmetalloproteinase9activationismediatedbysrcfamilytyrosinekinasewhichleadstoglialcelllinederivedneurotrophicfactormrnaexpressioninratastroglialcells |