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Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells

BACKGROUND: Astrocytes have been implicated in the pathophysiology of mood disorders and in the mechanism of the pharmacological effects of antidepressant drugs by the production of neurotrophic/growth factors. Previous studies have identified astrocyte‐expressed Gα(i/o)‐coupled lysophosphatidic aci...

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Autores principales: Abe, Hiromi, Kajitani, Naoto, Okada‐Tsuchioka, Mami, Omori, Wataru, Yatsumoto, Masahide, Takebayashi, Minoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292280/
https://www.ncbi.nlm.nih.gov/pubmed/31025529
http://dx.doi.org/10.1002/npr2.12055
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author Abe, Hiromi
Kajitani, Naoto
Okada‐Tsuchioka, Mami
Omori, Wataru
Yatsumoto, Masahide
Takebayashi, Minoru
author_facet Abe, Hiromi
Kajitani, Naoto
Okada‐Tsuchioka, Mami
Omori, Wataru
Yatsumoto, Masahide
Takebayashi, Minoru
author_sort Abe, Hiromi
collection PubMed
description BACKGROUND: Astrocytes have been implicated in the pathophysiology of mood disorders and in the mechanism of the pharmacological effects of antidepressant drugs by the production of neurotrophic/growth factors. Previous studies have identified astrocyte‐expressed Gα(i/o)‐coupled lysophosphatidic acid receptor 1 (LPAR1), as being involved in antidepressant‐induced production of glial cell line‐derived neurotrophic factor (GDNF) and matrix metalloproteinase‐9 (MMP‐9) activation, an important step in the production of GNDF. However, the precise mechanism of MMP‐9 activation by antidepressants has yet to be identified, in particular the intracellular signaling pathway between LPAR1/Gα(i/o) and MMP‐9. METHODS AND RESULTS: Treatment of rat C6 astroglial cells (C6 cells) with amitriptyline increased Src family tyrosine kinase phosphorylation in a time and concentration‐dependent manner. Amitriptyline‐induced GDNF mRNA expression was blocked by Src family tyrosine kinase inhibitors. In addition, inhibiting Src family tyrosine kinase blocked amitriptyline‐induced zymographic MMP‐9 activation in C6 cells. The amitriptyline‐induced zymographic MMP‐9 activity was completely blocked by selective inhibition of Gα(i/o) protein and LPAR1. Furthermore, the amitriptyline‐induced Src family tyrosine kinase phosphorylation was blocked by LPAR1, but not MMP‐9 inhibition, indicating that Src family tyrosine kinase involvement is downstream of LPAR1. CONCLUSIONS: The current findings suggest that the pharmacological effect of antidepressant such as amitriptyline is mediated through an intracellular signaling pathway via the LPAR1/Gα(i/o)/Src family tyrosine kinase, which leads to MMP‐9 activation and GDNF production.
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spelling pubmed-72922802020-12-08 Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells Abe, Hiromi Kajitani, Naoto Okada‐Tsuchioka, Mami Omori, Wataru Yatsumoto, Masahide Takebayashi, Minoru Neuropsychopharmacol Rep Original Articles BACKGROUND: Astrocytes have been implicated in the pathophysiology of mood disorders and in the mechanism of the pharmacological effects of antidepressant drugs by the production of neurotrophic/growth factors. Previous studies have identified astrocyte‐expressed Gα(i/o)‐coupled lysophosphatidic acid receptor 1 (LPAR1), as being involved in antidepressant‐induced production of glial cell line‐derived neurotrophic factor (GDNF) and matrix metalloproteinase‐9 (MMP‐9) activation, an important step in the production of GNDF. However, the precise mechanism of MMP‐9 activation by antidepressants has yet to be identified, in particular the intracellular signaling pathway between LPAR1/Gα(i/o) and MMP‐9. METHODS AND RESULTS: Treatment of rat C6 astroglial cells (C6 cells) with amitriptyline increased Src family tyrosine kinase phosphorylation in a time and concentration‐dependent manner. Amitriptyline‐induced GDNF mRNA expression was blocked by Src family tyrosine kinase inhibitors. In addition, inhibiting Src family tyrosine kinase blocked amitriptyline‐induced zymographic MMP‐9 activation in C6 cells. The amitriptyline‐induced zymographic MMP‐9 activity was completely blocked by selective inhibition of Gα(i/o) protein and LPAR1. Furthermore, the amitriptyline‐induced Src family tyrosine kinase phosphorylation was blocked by LPAR1, but not MMP‐9 inhibition, indicating that Src family tyrosine kinase involvement is downstream of LPAR1. CONCLUSIONS: The current findings suggest that the pharmacological effect of antidepressant such as amitriptyline is mediated through an intracellular signaling pathway via the LPAR1/Gα(i/o)/Src family tyrosine kinase, which leads to MMP‐9 activation and GDNF production. John Wiley and Sons Inc. 2019-04-25 /pmc/articles/PMC7292280/ /pubmed/31025529 http://dx.doi.org/10.1002/npr2.12055 Text en © 2019 The Authors. Neuropsychopharmacology Reports published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Neuropsychopharmacology This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Abe, Hiromi
Kajitani, Naoto
Okada‐Tsuchioka, Mami
Omori, Wataru
Yatsumoto, Masahide
Takebayashi, Minoru
Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells
title Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells
title_full Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells
title_fullStr Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells
title_full_unstemmed Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells
title_short Antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by Src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mRNA expression in rat astroglial cells
title_sort antidepressant amitriptyline‐induced matrix metalloproteinase‐9 activation is mediated by src family tyrosine kinase, which leads to glial cell line‐derived neurotrophic factor mrna expression in rat astroglial cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292280/
https://www.ncbi.nlm.nih.gov/pubmed/31025529
http://dx.doi.org/10.1002/npr2.12055
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