Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins

Site-saturation libraries reduce protein screening effort in directed evolution campaigns by focusing on a limited number of rationally chosen residues. However, uneven library synthesis efficiency leads to amino acid bias, remedied at high cost by expensive custom synthesis of oligonucleotides, or...

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Detalles Bibliográficos
Autores principales: Lindenburg, Laurens, Huovinen, Tuomas, van de Wiel, Kayleigh, Herger, Michael, Snaith, Michael R, Hollfelder, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293038/
https://www.ncbi.nlm.nih.gov/pubmed/32383757
http://dx.doi.org/10.1093/nar/gkaa270

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293038/
https://www.ncbi.nlm.nih.gov/pubmed/32383757
http://dx.doi.org/10.1093/nar/gkaa270

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