Cargando…
Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins
Site-saturation libraries reduce protein screening effort in directed evolution campaigns by focusing on a limited number of rationally chosen residues. However, uneven library synthesis efficiency leads to amino acid bias, remedied at high cost by expensive custom synthesis of oligonucleotides, or...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2020
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293038/ https://www.ncbi.nlm.nih.gov/pubmed/32383757 http://dx.doi.org/10.1093/nar/gkaa270 |