Massively parallel Cas13 screens reveal principles for guide RNA design

Type VI CRISPR enzymes are RNA-targeting proteins with nuclease activity that enable specific and robust target gene knock-down without altering the genome. To define rules for the design of Cas13d guide RNAs, we conducted massively-parallel screens targeting mRNAs of a green fluorescent protein (GF...

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Detalles Bibliográficos
Autores principales: Wessels, Hans-Hermann, Méndez-Mancilla, Alejandro, Guo, Xinyi, Legut, Mateusz, Daniloski, Zharko, Sanjana, Neville E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294996/
https://www.ncbi.nlm.nih.gov/pubmed/32518401
http://dx.doi.org/10.1038/s41587-020-0456-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294996/
https://www.ncbi.nlm.nih.gov/pubmed/32518401
http://dx.doi.org/10.1038/s41587-020-0456-9

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