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Massively parallel Cas13 screens reveal principles for guide RNA design
Type VI CRISPR enzymes are RNA-targeting proteins with nuclease activity that enable specific and robust target gene knock-down without altering the genome. To define rules for the design of Cas13d guide RNAs, we conducted massively-parallel screens targeting mRNAs of a green fluorescent protein (GF...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294996/ https://www.ncbi.nlm.nih.gov/pubmed/32518401 http://dx.doi.org/10.1038/s41587-020-0456-9 |