SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds

We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase target sites to generate multiplexed base editin...

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Detalles Bibliográficos
Autores principales: Li, Chao, Zong, Yuan, Jin, Shuai, Zhu, Haocheng, Lin, Dexing, Li, Shengnan, Qiu, Jin-Long, Wang, Yanpeng, Gao, Caixia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296638/
https://www.ncbi.nlm.nih.gov/pubmed/32546280
http://dx.doi.org/10.1186/s13059-020-02051-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296638/
https://www.ncbi.nlm.nih.gov/pubmed/32546280
http://dx.doi.org/10.1186/s13059-020-02051-x

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