A rapid and simple quantitative method for specific detection of smaller coterminal RNA by PCR (DeSCo-PCR): application to the detection of viral subgenomic RNAs

RNAs that are 5′-truncated versions of a longer RNA but share the same 3′ terminus can be generated by alternative promoters in transcription of cellular mRNAs or by replicating RNA viruses. These truncated RNAs cannot be distinguished from the longer RNA by a simple two-primer RT-PCR because primer...

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Detalles Bibliográficos
Autores principales: Kanodia, Pulkit, Prasanth, K. Reddisiva, Roa-Linares, Vicky C., Bradrick, Shelton S., Garcia-Blanco, Mariano A., Miller, W. Allen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297113/
https://www.ncbi.nlm.nih.gov/pubmed/32238481
http://dx.doi.org/10.1261/rna.074963.120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297113/
https://www.ncbi.nlm.nih.gov/pubmed/32238481
http://dx.doi.org/10.1261/rna.074963.120

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