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Real-time quantitative PCR assay development and application for assessment of agricultural surface water and various fecal matter for prevalence of Aliarcobacter faecis and Aliarcobacter lanthieri

BACKGROUND: Aliarcobacter faecis and Aliarcobacter lanthieri are recently identified as emerging human and animal pathogens. In this paper, we demonstrate the development and optimization of two direct DNA-based quantitative real-time PCR assays using species-specific oligonucleotide primer pairs de...

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Detalles Bibliográficos
Autores principales: Miltenburg, Mary G., Cloutier, Michel, Craiovan, Emilia, Lapen, David R., Wilkes, Graham, Topp, Edward, Khan, Izhar U. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7298852/
https://www.ncbi.nlm.nih.gov/pubmed/32546238
http://dx.doi.org/10.1186/s12866-020-01826-3