Investigation of anomalous charge variant profile reveals discrete pH-dependent conformations and conformation-dependent charge states within the CDR3 loop of a therapeutic mAb

During the development of a therapeutic monoclonal antibody (mAb-1), the charge variant profile obtained by pH-gradient cation exchange chromatography (CEX) contained two main peaks, each of which exhibited a unique intrinsic fluorescence profile and demonstrated inter-convertibility upon reinjectio...

Descripción completa

Detalles Bibliográficos
Autores principales: Lan, Wenkui, Valente, Joseph J., Ilott, Andrew, Chennamsetty, Naresh, Liu, Zhihua, Rizzo, Joseph M., Yamniuk, Aaron P., Qiu, Difei, Shackman, Holly M., Bolgar, Mark S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299213/
https://www.ncbi.nlm.nih.gov/pubmed/32432964
http://dx.doi.org/10.1080/19420862.2020.1763138

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299213/
https://www.ncbi.nlm.nih.gov/pubmed/32432964
http://dx.doi.org/10.1080/19420862.2020.1763138

Ejemplares similares