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Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies
Antibodies targeting the spike protein of SARS-CoV-2 present a promising approach to combat the COVID19 pandemic; however, concerns remain that mutations can yield antibody resistance. We investigate the development of resistance against four antibodies to the spike protein that potently neutralize...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299283/ https://www.ncbi.nlm.nih.gov/pubmed/32540904 http://dx.doi.org/10.1126/science.abd0831 |
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author | Baum, Alina Fulton, Benjamin O. Wloga, Elzbieta Copin, Richard Pascal, Kristen E. Russo, Vincenzo Giordano, Stephanie Lanza, Kathryn Negron, Nicole Ni, Min Wei, Yi Atwal, Gurinder S. Murphy, Andrew J. Stahl, Neil Yancopoulos, George D. Kyratsous, Christos A. |
author_facet | Baum, Alina Fulton, Benjamin O. Wloga, Elzbieta Copin, Richard Pascal, Kristen E. Russo, Vincenzo Giordano, Stephanie Lanza, Kathryn Negron, Nicole Ni, Min Wei, Yi Atwal, Gurinder S. Murphy, Andrew J. Stahl, Neil Yancopoulos, George D. Kyratsous, Christos A. |
author_sort | Baum, Alina |
collection | PubMed |
description | Antibodies targeting the spike protein of SARS-CoV-2 present a promising approach to combat the COVID19 pandemic; however, concerns remain that mutations can yield antibody resistance. We investigate the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails. These antibodies remain effective against spike variants that have arisen in the human population. However, novel spike mutants rapidly appeared following in vitro passaging in the presence of individual antibodies, resulting in loss of neutralization; such escape also occurred with combinations of antibodies binding diverse but overlapping regions of the spike protein. Importantly, escape mutants were not generated following treatment with a non-competing antibody cocktail. |
format | Online Article Text |
id | pubmed-7299283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72992832020-06-29 Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies Baum, Alina Fulton, Benjamin O. Wloga, Elzbieta Copin, Richard Pascal, Kristen E. Russo, Vincenzo Giordano, Stephanie Lanza, Kathryn Negron, Nicole Ni, Min Wei, Yi Atwal, Gurinder S. Murphy, Andrew J. Stahl, Neil Yancopoulos, George D. Kyratsous, Christos A. Science Reports Antibodies targeting the spike protein of SARS-CoV-2 present a promising approach to combat the COVID19 pandemic; however, concerns remain that mutations can yield antibody resistance. We investigate the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails. These antibodies remain effective against spike variants that have arisen in the human population. However, novel spike mutants rapidly appeared following in vitro passaging in the presence of individual antibodies, resulting in loss of neutralization; such escape also occurred with combinations of antibodies binding diverse but overlapping regions of the spike protein. Importantly, escape mutants were not generated following treatment with a non-competing antibody cocktail. American Association for the Advancement of Science 2020-06-15 /pmc/articles/PMC7299283/ /pubmed/32540904 http://dx.doi.org/10.1126/science.abd0831 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). http://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Reports Baum, Alina Fulton, Benjamin O. Wloga, Elzbieta Copin, Richard Pascal, Kristen E. Russo, Vincenzo Giordano, Stephanie Lanza, Kathryn Negron, Nicole Ni, Min Wei, Yi Atwal, Gurinder S. Murphy, Andrew J. Stahl, Neil Yancopoulos, George D. Kyratsous, Christos A. Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies |
title | Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies |
title_full | Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies |
title_fullStr | Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies |
title_full_unstemmed | Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies |
title_short | Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies |
title_sort | antibody cocktail to sars-cov-2 spike protein prevents rapid mutational escape seen with individual antibodies |
topic | Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299283/ https://www.ncbi.nlm.nih.gov/pubmed/32540904 http://dx.doi.org/10.1126/science.abd0831 |
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