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The effect of Sirt1 deficiency on Ca(2+) and Na(+) regulation in mouse ventricular myocytes

This study addressed the hypothesis that cardiac Sirtuin 1 (Sirt1) deficiency alters cardiomyocyte Ca(2+) and Na(+) regulation, leading to cardiac dysfunction and arrhythmogenesis. We used mice with cardiac‐specific Sirt1 knockout (Sirt1(−/−)). Sirt1(flox/flox) mice were served as control. Sirt1(−/−...

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Autores principales: Yang, Hsiang‐Yu, Lin, Feng‐Zhi, Yang, Hui‐Wen, Yu, Pei‐Ling, Huang, Shih‐Ming, Chen, Yao‐Chang, Tsai, Chien‐Sung, Lin, Chih‐Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299725/
https://www.ncbi.nlm.nih.gov/pubmed/32342656
http://dx.doi.org/10.1111/jcmm.15327
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author Yang, Hsiang‐Yu
Lin, Feng‐Zhi
Yang, Hui‐Wen
Yu, Pei‐Ling
Huang, Shih‐Ming
Chen, Yao‐Chang
Tsai, Chien‐Sung
Lin, Chih‐Yuan
author_facet Yang, Hsiang‐Yu
Lin, Feng‐Zhi
Yang, Hui‐Wen
Yu, Pei‐Ling
Huang, Shih‐Ming
Chen, Yao‐Chang
Tsai, Chien‐Sung
Lin, Chih‐Yuan
author_sort Yang, Hsiang‐Yu
collection PubMed
description This study addressed the hypothesis that cardiac Sirtuin 1 (Sirt1) deficiency alters cardiomyocyte Ca(2+) and Na(+) regulation, leading to cardiac dysfunction and arrhythmogenesis. We used mice with cardiac‐specific Sirt1 knockout (Sirt1(−/−)). Sirt1(flox/flox) mice were served as control. Sirt1(−/−) mice showed impaired cardiac ejection fraction with increased ventricular spontaneous activity and burst firing compared with those in control mice. The arrhythmic events were suppressed by KN93 and ranolazine. Reduction in Ca(2+) transient amplitudes and sarcoplasmic reticulum (SR) Ca(2+) stores, and increased SR Ca(2+) leak were shown in the Sirt1(−/−) mice. Electrophysiological measurements were performed using patch‐clamp method. While L‐type Ca(2+) current (I (Ca, L)) was smaller in Sirt1(−/−) myocytes, reverse‐mode Na(+)/Ca(2+) exchanger (NCX) current was larger compared with those in control myocytes. Late Na(+) current (I (Na, L)) was enhanced in the Sirt1(−/−) mice, alongside with elevated cytosolic Na(+) level. Increased cytosolic and mitochondrial reactive oxygen species (ROS) were shown in Sirt1(−/−) mice. Sirt1(−/−) cardiomyocytes showed down‐regulation of L‐type Ca(2+) channel α1c subunit (Cav1.2) and sarcoplasmic/endoplasmic reticulum Ca(2+) ATPase 2a (SERCA2a), but up‐regulation of Ca(2+)/calmodulin‐dependent protein kinase II and NCX. In conclusions, these findings suggest that deficiency of Sirt1 impairs the regulation of intracellular Ca(2+) and Na(+) in cardiomyocytes, thereby provoking cardiac dysfunction and arrhythmogenesis.
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spelling pubmed-72997252020-06-18 The effect of Sirt1 deficiency on Ca(2+) and Na(+) regulation in mouse ventricular myocytes Yang, Hsiang‐Yu Lin, Feng‐Zhi Yang, Hui‐Wen Yu, Pei‐Ling Huang, Shih‐Ming Chen, Yao‐Chang Tsai, Chien‐Sung Lin, Chih‐Yuan J Cell Mol Med Original Articles This study addressed the hypothesis that cardiac Sirtuin 1 (Sirt1) deficiency alters cardiomyocyte Ca(2+) and Na(+) regulation, leading to cardiac dysfunction and arrhythmogenesis. We used mice with cardiac‐specific Sirt1 knockout (Sirt1(−/−)). Sirt1(flox/flox) mice were served as control. Sirt1(−/−) mice showed impaired cardiac ejection fraction with increased ventricular spontaneous activity and burst firing compared with those in control mice. The arrhythmic events were suppressed by KN93 and ranolazine. Reduction in Ca(2+) transient amplitudes and sarcoplasmic reticulum (SR) Ca(2+) stores, and increased SR Ca(2+) leak were shown in the Sirt1(−/−) mice. Electrophysiological measurements were performed using patch‐clamp method. While L‐type Ca(2+) current (I (Ca, L)) was smaller in Sirt1(−/−) myocytes, reverse‐mode Na(+)/Ca(2+) exchanger (NCX) current was larger compared with those in control myocytes. Late Na(+) current (I (Na, L)) was enhanced in the Sirt1(−/−) mice, alongside with elevated cytosolic Na(+) level. Increased cytosolic and mitochondrial reactive oxygen species (ROS) were shown in Sirt1(−/−) mice. Sirt1(−/−) cardiomyocytes showed down‐regulation of L‐type Ca(2+) channel α1c subunit (Cav1.2) and sarcoplasmic/endoplasmic reticulum Ca(2+) ATPase 2a (SERCA2a), but up‐regulation of Ca(2+)/calmodulin‐dependent protein kinase II and NCX. In conclusions, these findings suggest that deficiency of Sirt1 impairs the regulation of intracellular Ca(2+) and Na(+) in cardiomyocytes, thereby provoking cardiac dysfunction and arrhythmogenesis. John Wiley and Sons Inc. 2020-04-27 2020-06 /pmc/articles/PMC7299725/ /pubmed/32342656 http://dx.doi.org/10.1111/jcmm.15327 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Yang, Hsiang‐Yu
Lin, Feng‐Zhi
Yang, Hui‐Wen
Yu, Pei‐Ling
Huang, Shih‐Ming
Chen, Yao‐Chang
Tsai, Chien‐Sung
Lin, Chih‐Yuan
The effect of Sirt1 deficiency on Ca(2+) and Na(+) regulation in mouse ventricular myocytes
title The effect of Sirt1 deficiency on Ca(2+) and Na(+) regulation in mouse ventricular myocytes
title_full The effect of Sirt1 deficiency on Ca(2+) and Na(+) regulation in mouse ventricular myocytes
title_fullStr The effect of Sirt1 deficiency on Ca(2+) and Na(+) regulation in mouse ventricular myocytes
title_full_unstemmed The effect of Sirt1 deficiency on Ca(2+) and Na(+) regulation in mouse ventricular myocytes
title_short The effect of Sirt1 deficiency on Ca(2+) and Na(+) regulation in mouse ventricular myocytes
title_sort effect of sirt1 deficiency on ca(2+) and na(+) regulation in mouse ventricular myocytes
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299725/
https://www.ncbi.nlm.nih.gov/pubmed/32342656
http://dx.doi.org/10.1111/jcmm.15327
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