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Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0

CRISPR/Cas9 systems are an established tool in genome engineering. As double strand breaks caused by the standard Cas9-based knock-out techniques can be problematic in some organisms, new systems were developed that can efficiently create knock-outs without causing double strand breaks to elegantly...

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Detalles Bibliográficos
Autores principales: Blin, Kai, Shaw, Simon, Tong, Yaojun, Weber, Tilmann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301206/
https://www.ncbi.nlm.nih.gov/pubmed/32596519
http://dx.doi.org/10.1016/j.synbio.2020.05.005