Cargando…
Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0
CRISPR/Cas9 systems are an established tool in genome engineering. As double strand breaks caused by the standard Cas9-based knock-out techniques can be problematic in some organisms, new systems were developed that can efficiently create knock-outs without causing double strand breaks to elegantly...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301206/ https://www.ncbi.nlm.nih.gov/pubmed/32596519 http://dx.doi.org/10.1016/j.synbio.2020.05.005 |