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High-Throughput Fluorescence Assay for Ketone Detection and Its Applications in Enzyme Mining and Protein Engineering

[Image: see text] Ketones are of great importance as building blocks in synthetic organic chemistry and biocatalysis. Most ketones cannot easily be quantitatively assayed due to the lack of visible photometric properties. Effective high-throughput assay (HTA) development is therefore necessary for k...

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Detalles Bibliográficos
Autores principales: Mei, Zelong, Zhang, Kun, Qu, Ge, Li, Jun-Kuan, Liu, Baoyan, Ma, Jun-An, Tu, Ran, Sun, Zhoutong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301380/
https://www.ncbi.nlm.nih.gov/pubmed/32566823
http://dx.doi.org/10.1021/acsomega.0c00245