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Preparation of a phosphotyrosine-protein standard for use in semiquantitative western blotting with enhanced chemiluminescence

Protein tyrosine phosphorylation is key to activation of receptor tyrosine kinases (RTK) that drive development of some cancers. One challenge of RTK-targeted therapy is identification of those tumors that express non-mutated but activated RTKs. Phosphotyrosine (pTyr) RTK levels should be more predi...

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Detalles Bibliográficos
Autores principales: Kendrick, Nancy, Powers, Ginny, Johansen, Jon, Hoelter, Matt, Koll, Andrew, Carlson, Sofia, Channaveerappa, Devika, Darie, Costel C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302481/
https://www.ncbi.nlm.nih.gov/pubmed/32555693
http://dx.doi.org/10.1371/journal.pone.0234645