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Extraction and reconstitution of membrane proteins into lipid nanodiscs encased by zwitterionic styrene-maleic amide copolymers

Membrane proteins can be reconstituted in polymer-encased nanodiscs for studies under near-physiological conditions and in the absence of detergents, but traditional styrene-maleic acid copolymers used for this purpose suffer severely from buffer incompatibilities. We have recently introduced zwitte...

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Detalles Bibliográficos
Autores principales: Fiori, Mariana C., Zheng, Wan, Kamilar, Elizabeth, Simiyu, Geuel, Altenberg, Guillermo A., Liang, Hongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303149/
https://www.ncbi.nlm.nih.gov/pubmed/32555261
http://dx.doi.org/10.1038/s41598-020-66852-7