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Extraction and reconstitution of membrane proteins into lipid nanodiscs encased by zwitterionic styrene-maleic amide copolymers
Membrane proteins can be reconstituted in polymer-encased nanodiscs for studies under near-physiological conditions and in the absence of detergents, but traditional styrene-maleic acid copolymers used for this purpose suffer severely from buffer incompatibilities. We have recently introduced zwitte...
Autores principales: | Fiori, Mariana C., Zheng, Wan, Kamilar, Elizabeth, Simiyu, Geuel, Altenberg, Guillermo A., Liang, Hongjun |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303149/ https://www.ncbi.nlm.nih.gov/pubmed/32555261 http://dx.doi.org/10.1038/s41598-020-66852-7 |
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