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Highly efficient synchronization of sheep skin fibroblasts at G2/M phase and isolation of sheep Y chromosomes by flow cytometric sorting

At present, based on whole genome sequencing, sequences and genes annotation of the sheep (Ovis aries) Y chromosome are still absent. The isolation of Y chromosomes followed by sequencing has been approved as an effective approach to analyze this complex chromosome in other species. In this study, w...

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Detalles Bibliográficos
Autores principales: Yao, Yanzhu, Zhang, Yuanyuan, Liu, Wansheng, Deng, Xuemei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303189/
https://www.ncbi.nlm.nih.gov/pubmed/32555328
http://dx.doi.org/10.1038/s41598-020-66905-x