Cargando…
Cryo-EM structure of the nonameric CsgG-CsgF complex and its implications for controlling curli biogenesis in Enterobacteriaceae
Curli play critical roles in biofilm formation, host cell adhesion, and colonization of inert surfaces in many Enterobacteriaceae. In Escherichia coli, curli biogenesis requires 7 curli-specific gene (csg) products—CsgA through G—working in concert. Of them, CsgG and CsgF are 2 outer membrane (OM)-l...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304575/ https://www.ncbi.nlm.nih.gov/pubmed/32559189 http://dx.doi.org/10.1371/journal.pbio.3000748 |
_version_ | 1783548281291276288 |
---|---|
author | Zhang, Manfeng Shi, Huigang Zhang, Xuemei Zhang, Xinzheng Huang, Yihua |
author_facet | Zhang, Manfeng Shi, Huigang Zhang, Xuemei Zhang, Xinzheng Huang, Yihua |
author_sort | Zhang, Manfeng |
collection | PubMed |
description | Curli play critical roles in biofilm formation, host cell adhesion, and colonization of inert surfaces in many Enterobacteriaceae. In Escherichia coli, curli biogenesis requires 7 curli-specific gene (csg) products—CsgA through G—working in concert. Of them, CsgG and CsgF are 2 outer membrane (OM)-localized components that consists of the core apparatus for secretion and assembly of curli structural subunits, CsgB and CsgA. Here, we report the cryogenic electron microscopy (cryo-EM) structure of CsgG in complex with CsgF from E. coli. The structure reveals that CsgF forms a stable complex with CsgG via a 1:1 stoichiometry by lining the upper lumen of the nonameric CsgG channel via its N-terminal 27 residues, forming a funnel-like entity plugged in the CsgG channel and creating a unique secretion channel with 2 constriction regions, consistent with the recently reported structure of the CsgG-CsgF complex. Functional studies indicate that export of CsgF to the cell surface requires the CsgG channel, and CsgF not only functions as an adaptor that bridges CsgB with CsgG but also may play important roles in controlling the rates of translocation and/or polymerization for curli structural subunits. Importantly, we found that a series of CsgF-derived peptides are able to efficiently inhibit curli production to E. coli when administrated exogenously, highlighting a potential strategy to interfere biofilm formation in E. coli strains. |
format | Online Article Text |
id | pubmed-7304575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-73045752020-06-19 Cryo-EM structure of the nonameric CsgG-CsgF complex and its implications for controlling curli biogenesis in Enterobacteriaceae Zhang, Manfeng Shi, Huigang Zhang, Xuemei Zhang, Xinzheng Huang, Yihua PLoS Biol Research Article Curli play critical roles in biofilm formation, host cell adhesion, and colonization of inert surfaces in many Enterobacteriaceae. In Escherichia coli, curli biogenesis requires 7 curli-specific gene (csg) products—CsgA through G—working in concert. Of them, CsgG and CsgF are 2 outer membrane (OM)-localized components that consists of the core apparatus for secretion and assembly of curli structural subunits, CsgB and CsgA. Here, we report the cryogenic electron microscopy (cryo-EM) structure of CsgG in complex with CsgF from E. coli. The structure reveals that CsgF forms a stable complex with CsgG via a 1:1 stoichiometry by lining the upper lumen of the nonameric CsgG channel via its N-terminal 27 residues, forming a funnel-like entity plugged in the CsgG channel and creating a unique secretion channel with 2 constriction regions, consistent with the recently reported structure of the CsgG-CsgF complex. Functional studies indicate that export of CsgF to the cell surface requires the CsgG channel, and CsgF not only functions as an adaptor that bridges CsgB with CsgG but also may play important roles in controlling the rates of translocation and/or polymerization for curli structural subunits. Importantly, we found that a series of CsgF-derived peptides are able to efficiently inhibit curli production to E. coli when administrated exogenously, highlighting a potential strategy to interfere biofilm formation in E. coli strains. Public Library of Science 2020-06-19 /pmc/articles/PMC7304575/ /pubmed/32559189 http://dx.doi.org/10.1371/journal.pbio.3000748 Text en © 2020 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhang, Manfeng Shi, Huigang Zhang, Xuemei Zhang, Xinzheng Huang, Yihua Cryo-EM structure of the nonameric CsgG-CsgF complex and its implications for controlling curli biogenesis in Enterobacteriaceae |
title | Cryo-EM structure of the nonameric CsgG-CsgF complex and its implications for controlling curli biogenesis in Enterobacteriaceae |
title_full | Cryo-EM structure of the nonameric CsgG-CsgF complex and its implications for controlling curli biogenesis in Enterobacteriaceae |
title_fullStr | Cryo-EM structure of the nonameric CsgG-CsgF complex and its implications for controlling curli biogenesis in Enterobacteriaceae |
title_full_unstemmed | Cryo-EM structure of the nonameric CsgG-CsgF complex and its implications for controlling curli biogenesis in Enterobacteriaceae |
title_short | Cryo-EM structure of the nonameric CsgG-CsgF complex and its implications for controlling curli biogenesis in Enterobacteriaceae |
title_sort | cryo-em structure of the nonameric csgg-csgf complex and its implications for controlling curli biogenesis in enterobacteriaceae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304575/ https://www.ncbi.nlm.nih.gov/pubmed/32559189 http://dx.doi.org/10.1371/journal.pbio.3000748 |
work_keys_str_mv | AT zhangmanfeng cryoemstructureofthenonamericcsggcsgfcomplexanditsimplicationsforcontrollingcurlibiogenesisinenterobacteriaceae AT shihuigang cryoemstructureofthenonamericcsggcsgfcomplexanditsimplicationsforcontrollingcurlibiogenesisinenterobacteriaceae AT zhangxuemei cryoemstructureofthenonamericcsggcsgfcomplexanditsimplicationsforcontrollingcurlibiogenesisinenterobacteriaceae AT zhangxinzheng cryoemstructureofthenonamericcsggcsgfcomplexanditsimplicationsforcontrollingcurlibiogenesisinenterobacteriaceae AT huangyihua cryoemstructureofthenonamericcsggcsgfcomplexanditsimplicationsforcontrollingcurlibiogenesisinenterobacteriaceae |