Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

With the rise of new powerful genome engineering technologies, such as CRISPR/Cas9, cell models can be engineered effectively to accelerate basic and disease research. The most critical step in this procedure is the efficient delivery of foreign nucleic acids into cells by cellular transfection. Sin...

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Detalles Bibliográficos
Autores principales: Søndergaard, Jonas Nørskov, Geng, Keyi, Sommerauer, Christian, Atanasoai, Ionut, Yin, Xiushan, Kutter, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305135/
https://www.ncbi.nlm.nih.gov/pubmed/32561814
http://dx.doi.org/10.1038/s42003-020-1045-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305135/
https://www.ncbi.nlm.nih.gov/pubmed/32561814
http://dx.doi.org/10.1038/s42003-020-1045-7

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