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Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids
With the rise of new powerful genome engineering technologies, such as CRISPR/Cas9, cell models can be engineered effectively to accelerate basic and disease research. The most critical step in this procedure is the efficient delivery of foreign nucleic acids into cells by cellular transfection. Sin...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305135/ https://www.ncbi.nlm.nih.gov/pubmed/32561814 http://dx.doi.org/10.1038/s42003-020-1045-7 |