Cargando…

Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis

We developed a label-free, real-time, and highly sensitive nucleic acid biosensor based on fiber optic particle plasmon resonance (FOPPR). The biosensor employs a single-strand deoxyoligonucleotides (ssDNA) probe, conjugated to immobilized gold nanoparticles on the core surface of an optical fiber....

Descripción completa

Detalles Bibliográficos
Autores principales: Tseng, Yen-Ta, Li, Wan-Yun, Yu, Ya-Wen, Chiang, Chang-Yue, Liu, Su-Qin, Chau, Lai-Kwan, Lai, Ning-Sheng, Chou, Cheng-Chung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309088/
https://www.ncbi.nlm.nih.gov/pubmed/32492975
http://dx.doi.org/10.3390/s20113137
_version_ 1783549143501766656
author Tseng, Yen-Ta
Li, Wan-Yun
Yu, Ya-Wen
Chiang, Chang-Yue
Liu, Su-Qin
Chau, Lai-Kwan
Lai, Ning-Sheng
Chou, Cheng-Chung
author_facet Tseng, Yen-Ta
Li, Wan-Yun
Yu, Ya-Wen
Chiang, Chang-Yue
Liu, Su-Qin
Chau, Lai-Kwan
Lai, Ning-Sheng
Chou, Cheng-Chung
author_sort Tseng, Yen-Ta
collection PubMed
description We developed a label-free, real-time, and highly sensitive nucleic acid biosensor based on fiber optic particle plasmon resonance (FOPPR). The biosensor employs a single-strand deoxyoligonucleotides (ssDNA) probe, conjugated to immobilized gold nanoparticles on the core surface of an optical fiber. We explore the steric effects on hybridization affinity and limit of detection (LOD), by using different ssDNA probe designs and surface chemistries, including diluent molecules of different lengths in mixed self-assembled monolayers, ssDNA probes of different oligonucleotide lengths, ssDNA probes in different orientations to accommodate target oligonucleotides with a hybridization region located unevenly in the strand. Based on the optimized ssDNA probe design and surface chemistry, we achieved LOD at sub-nM level, which makes detection of target oligonucleotides as low as 1 fmol possible in the 10-μL sensor chip. Additionally, the FOPPR biosensor shows a good correlation in determining HLA-B27 mRNA, in extracted blood samples from patients with ankylosing spondylitis (AS), with the clinically accepted real-time reverse transcription-polymerase chain reaction (RT-PCR) method. The results from this fundamental study should guide the design of ssDNA probe for anti-sense sensing. Further results through application to HLA-B27 mRNA detection illustrate the feasibility in detecting various nucleic acids of chemical and biological relevance.
format Online
Article
Text
id pubmed-7309088
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73090882020-06-25 Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis Tseng, Yen-Ta Li, Wan-Yun Yu, Ya-Wen Chiang, Chang-Yue Liu, Su-Qin Chau, Lai-Kwan Lai, Ning-Sheng Chou, Cheng-Chung Sensors (Basel) Article We developed a label-free, real-time, and highly sensitive nucleic acid biosensor based on fiber optic particle plasmon resonance (FOPPR). The biosensor employs a single-strand deoxyoligonucleotides (ssDNA) probe, conjugated to immobilized gold nanoparticles on the core surface of an optical fiber. We explore the steric effects on hybridization affinity and limit of detection (LOD), by using different ssDNA probe designs and surface chemistries, including diluent molecules of different lengths in mixed self-assembled monolayers, ssDNA probes of different oligonucleotide lengths, ssDNA probes in different orientations to accommodate target oligonucleotides with a hybridization region located unevenly in the strand. Based on the optimized ssDNA probe design and surface chemistry, we achieved LOD at sub-nM level, which makes detection of target oligonucleotides as low as 1 fmol possible in the 10-μL sensor chip. Additionally, the FOPPR biosensor shows a good correlation in determining HLA-B27 mRNA, in extracted blood samples from patients with ankylosing spondylitis (AS), with the clinically accepted real-time reverse transcription-polymerase chain reaction (RT-PCR) method. The results from this fundamental study should guide the design of ssDNA probe for anti-sense sensing. Further results through application to HLA-B27 mRNA detection illustrate the feasibility in detecting various nucleic acids of chemical and biological relevance. MDPI 2020-06-01 /pmc/articles/PMC7309088/ /pubmed/32492975 http://dx.doi.org/10.3390/s20113137 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tseng, Yen-Ta
Li, Wan-Yun
Yu, Ya-Wen
Chiang, Chang-Yue
Liu, Su-Qin
Chau, Lai-Kwan
Lai, Ning-Sheng
Chou, Cheng-Chung
Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis
title Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis
title_full Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis
title_fullStr Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis
title_full_unstemmed Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis
title_short Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis
title_sort fiber optic particle plasmon resonance biosensor for label-free detection of nucleic acids and its application to hla-b27 mrna detection in patients with ankylosing spondylitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309088/
https://www.ncbi.nlm.nih.gov/pubmed/32492975
http://dx.doi.org/10.3390/s20113137
work_keys_str_mv AT tsengyenta fiberopticparticleplasmonresonancebiosensorforlabelfreedetectionofnucleicacidsanditsapplicationtohlab27mrnadetectioninpatientswithankylosingspondylitis
AT liwanyun fiberopticparticleplasmonresonancebiosensorforlabelfreedetectionofnucleicacidsanditsapplicationtohlab27mrnadetectioninpatientswithankylosingspondylitis
AT yuyawen fiberopticparticleplasmonresonancebiosensorforlabelfreedetectionofnucleicacidsanditsapplicationtohlab27mrnadetectioninpatientswithankylosingspondylitis
AT chiangchangyue fiberopticparticleplasmonresonancebiosensorforlabelfreedetectionofnucleicacidsanditsapplicationtohlab27mrnadetectioninpatientswithankylosingspondylitis
AT liusuqin fiberopticparticleplasmonresonancebiosensorforlabelfreedetectionofnucleicacidsanditsapplicationtohlab27mrnadetectioninpatientswithankylosingspondylitis
AT chaulaikwan fiberopticparticleplasmonresonancebiosensorforlabelfreedetectionofnucleicacidsanditsapplicationtohlab27mrnadetectioninpatientswithankylosingspondylitis
AT lainingsheng fiberopticparticleplasmonresonancebiosensorforlabelfreedetectionofnucleicacidsanditsapplicationtohlab27mrnadetectioninpatientswithankylosingspondylitis
AT chouchengchung fiberopticparticleplasmonresonancebiosensorforlabelfreedetectionofnucleicacidsanditsapplicationtohlab27mrnadetectioninpatientswithankylosingspondylitis