A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system

BACKGROUND: Reverse genetics is used in many laboratories around the world and enables the creation of tailor-made influenza viruses with a desired genotype or phenotype. However, the process is not flawless, and difficulties remain during cloning of influenza gene segments into reverse genetics vec...

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Detalles Bibliográficos
Autores principales: Bhat, Sushant, Bialy, Dagmara, Sealy, Joshua E., Sadeyen, Jean-Remy, Chang, Pengxiang, Iqbal, Munir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309217/
https://www.ncbi.nlm.nih.gov/pubmed/32576218
http://dx.doi.org/10.1186/s12985-020-01358-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309217/
https://www.ncbi.nlm.nih.gov/pubmed/32576218
http://dx.doi.org/10.1186/s12985-020-01358-2

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