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FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells

In this study, we present a straightforward approach for functional cell-based screening by co-encapsulation of secretor yeast cells and reporter mammalian cells in millions of individual agarose-containing microdroplets. Our system is compatible with ultra-high-throughput selection utilizing standa...

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Detalles Bibliográficos
Autores principales: Yanakieva, Desislava, Elter, Adrian, Bratsch, Jens, Friedrich, Karlheinz, Becker, Stefan, Kolmar, Harald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311539/
https://www.ncbi.nlm.nih.gov/pubmed/32576855
http://dx.doi.org/10.1038/s41598-020-66927-5