Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 (DE3): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced

This study aimed to express heterologously the lipase LipA from Pseudomonas aeruginosa PSA01 obtained from palm fruit residues. In previous approaches, LipA was expressed in Escherichia coli fused with its signal peptide and without its disulfide bond, displaying low activity. We cloned the mature L...

Descripción completa

Detalles Bibliográficos
Autores principales: Pulido, Ingrid Yamile, Prieto, Erlide, Pieffet, Gilles Paul, Méndez, Lina, Jiménez-Junca, Carlos A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312249/
https://www.ncbi.nlm.nih.gov/pubmed/32486240
http://dx.doi.org/10.3390/ijms21113925

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312249/
https://www.ncbi.nlm.nih.gov/pubmed/32486240
http://dx.doi.org/10.3390/ijms21113925

Ejemplares similares