Fractionation of Enriched Phosphopeptides Using pH/Acetonitrile-Gradient-Reversed-Phase Microcolumn Separation in Combination with LC–MS/MS Analysis

Mass spectrometry (MS) is a powerful and sensitive method often used for the identification of phosphoproteins. However, in phosphoproteomics, there is an identified need to compensate for the low abundance, insufficient ionization, and suppression effects of non-phosphorylated peptides. These may h...

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Detalles Bibliográficos
Autores principales: Ondrej, Martin, Rehulka, Pavel, Rehulkova, Helena, Kupcik, Rudolf, Tichy, Ales
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312998/
https://www.ncbi.nlm.nih.gov/pubmed/32492839
http://dx.doi.org/10.3390/ijms21113971

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312998/
https://www.ncbi.nlm.nih.gov/pubmed/32492839
http://dx.doi.org/10.3390/ijms21113971

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