Combined Optimization of Codon Usage and Glycine Supplementation Enhances the Extracellular Production of a β-Cyclodextrin Glycosyltransferase from Bacillus sp. NR5 UPM in Escherichia coli

Two optimization strategies, codon usage modification and glycine supplementation, were adopted to improve the extracellular production of Bacillus sp. NR5 UPM β-cyclodextrin glycosyltransferase (CGT-BS) in recombinant Escherichia coli. Several rare codons were eliminated and replaced with the ones...

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Detalles Bibliográficos
Autores principales: Nik-Pa, Nik Ida Mardiana, Sobri, Mohamad Farhan Mohamad, Abd-Aziz, Suraini, Ibrahim, Mohamad Faizal, Kamal Bahrin, Ezyana, Mohammed Alitheen, Noorjahan Banu, Ramli, Norhayati
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313058/
https://www.ncbi.nlm.nih.gov/pubmed/32486212
http://dx.doi.org/10.3390/ijms21113919

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313058/
https://www.ncbi.nlm.nih.gov/pubmed/32486212
http://dx.doi.org/10.3390/ijms21113919

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