Cargando…

Rolling circle amplification of synthetic DNA accelerates biocatalytic determination of enzyme activity relative to conventional methods

The ability to quickly and easily assess the activity of large collections of enzymes for a desired substrate holds great promise in the field of biocatalysis. Cell-free synthesis, although not practically amenable for large-scale enzyme production, provides a way to accelerate the timeline for scre...

Descripción completa

Detalles Bibliográficos
Autores principales: Hadi, Timin, Nozzi, Nicole, Melby, Joel O., Gao, Wei, Fuerst, Douglas E., Kvam, Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314814/
https://www.ncbi.nlm.nih.gov/pubmed/32581345
http://dx.doi.org/10.1038/s41598-020-67307-9