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Rolling circle amplification of synthetic DNA accelerates biocatalytic determination of enzyme activity relative to conventional methods
The ability to quickly and easily assess the activity of large collections of enzymes for a desired substrate holds great promise in the field of biocatalysis. Cell-free synthesis, although not practically amenable for large-scale enzyme production, provides a way to accelerate the timeline for scre...
Autores principales: | Hadi, Timin, Nozzi, Nicole, Melby, Joel O., Gao, Wei, Fuerst, Douglas E., Kvam, Erik |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314814/ https://www.ncbi.nlm.nih.gov/pubmed/32581345 http://dx.doi.org/10.1038/s41598-020-67307-9 |
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