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Diversity and spoilage potential of microbial communities associated with grape sour rot in eastern coastal areas of China

As a polymicrobial disease, sour rot decreases grape berry yield and wine quality. The diversity of microbial communities in sour rot-affected grapes depends on the cultivation site, but the microbes responsible for this disease in eastern coastal China, has not been reported. To identify the microb...

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Autores principales: Gao, Huanhuan, Yin, Xiangtian, Jiang, Xilong, Shi, Hongmei, Yang, Yang, Wang, Chaoping, Dai, Xiaoyan, Chen, Yingchun, Wu, Xinying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315622/
https://www.ncbi.nlm.nih.gov/pubmed/32607286
http://dx.doi.org/10.7717/peerj.9376
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author Gao, Huanhuan
Yin, Xiangtian
Jiang, Xilong
Shi, Hongmei
Yang, Yang
Wang, Chaoping
Dai, Xiaoyan
Chen, Yingchun
Wu, Xinying
author_facet Gao, Huanhuan
Yin, Xiangtian
Jiang, Xilong
Shi, Hongmei
Yang, Yang
Wang, Chaoping
Dai, Xiaoyan
Chen, Yingchun
Wu, Xinying
author_sort Gao, Huanhuan
collection PubMed
description As a polymicrobial disease, sour rot decreases grape berry yield and wine quality. The diversity of microbial communities in sour rot-affected grapes depends on the cultivation site, but the microbes responsible for this disease in eastern coastal China, has not been reported. To identify the microbes that cause sour grape rot in this important grape-producing region, the diversity and abundance of bacteria and fungi were assessed by metagenomic analysis and cultivation-dependent techniques. A total of 15 bacteria and 10 fungi were isolated from sour rot-affected grapes. High-throughput sequencing of PCR-amplicons generated from diseased grapes revealed 1343 OTUs of bacteria and 1038 OTUs of fungi. Proteobacteria and Firmicutes were dominant phyla among the 19 bacterial phyla identified. Ascomycota was the dominant fungal phylum and the fungi Issatchenkia terricola, Colletotrichum viniferum, Hanseniaspora vineae, Saprochaete gigas, and Candida diversa represented the vast majority ofmicrobial species associated with sour rot-affected grapes. An in vitro spoilage assay confirmed that four of the isolated bacteria strains (two Cronobacter species, Serratia marcescens and Lysinibacillus fusiformis) and five of the isolated fungi strains (three Aspergillus species, Alternaria tenuissima, and Fusarium proliferatum) spoiled grapes. These microorganisms, which appear responsible for spoiling grapes in eastern China, appear closely related to microbes that cause this plant disease around the world.
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spelling pubmed-73156222020-06-29 Diversity and spoilage potential of microbial communities associated with grape sour rot in eastern coastal areas of China Gao, Huanhuan Yin, Xiangtian Jiang, Xilong Shi, Hongmei Yang, Yang Wang, Chaoping Dai, Xiaoyan Chen, Yingchun Wu, Xinying PeerJ Agricultural Science As a polymicrobial disease, sour rot decreases grape berry yield and wine quality. The diversity of microbial communities in sour rot-affected grapes depends on the cultivation site, but the microbes responsible for this disease in eastern coastal China, has not been reported. To identify the microbes that cause sour grape rot in this important grape-producing region, the diversity and abundance of bacteria and fungi were assessed by metagenomic analysis and cultivation-dependent techniques. A total of 15 bacteria and 10 fungi were isolated from sour rot-affected grapes. High-throughput sequencing of PCR-amplicons generated from diseased grapes revealed 1343 OTUs of bacteria and 1038 OTUs of fungi. Proteobacteria and Firmicutes were dominant phyla among the 19 bacterial phyla identified. Ascomycota was the dominant fungal phylum and the fungi Issatchenkia terricola, Colletotrichum viniferum, Hanseniaspora vineae, Saprochaete gigas, and Candida diversa represented the vast majority ofmicrobial species associated with sour rot-affected grapes. An in vitro spoilage assay confirmed that four of the isolated bacteria strains (two Cronobacter species, Serratia marcescens and Lysinibacillus fusiformis) and five of the isolated fungi strains (three Aspergillus species, Alternaria tenuissima, and Fusarium proliferatum) spoiled grapes. These microorganisms, which appear responsible for spoiling grapes in eastern China, appear closely related to microbes that cause this plant disease around the world. PeerJ Inc. 2020-06-16 /pmc/articles/PMC7315622/ /pubmed/32607286 http://dx.doi.org/10.7717/peerj.9376 Text en ©2020 Gao et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Gao, Huanhuan
Yin, Xiangtian
Jiang, Xilong
Shi, Hongmei
Yang, Yang
Wang, Chaoping
Dai, Xiaoyan
Chen, Yingchun
Wu, Xinying
Diversity and spoilage potential of microbial communities associated with grape sour rot in eastern coastal areas of China
title Diversity and spoilage potential of microbial communities associated with grape sour rot in eastern coastal areas of China
title_full Diversity and spoilage potential of microbial communities associated with grape sour rot in eastern coastal areas of China
title_fullStr Diversity and spoilage potential of microbial communities associated with grape sour rot in eastern coastal areas of China
title_full_unstemmed Diversity and spoilage potential of microbial communities associated with grape sour rot in eastern coastal areas of China
title_short Diversity and spoilage potential of microbial communities associated with grape sour rot in eastern coastal areas of China
title_sort diversity and spoilage potential of microbial communities associated with grape sour rot in eastern coastal areas of china
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315622/
https://www.ncbi.nlm.nih.gov/pubmed/32607286
http://dx.doi.org/10.7717/peerj.9376
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