Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes

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Biotin-labeled molecular probes, comprising specific regions of the SARS-CoV-2 spike, would be helpful in the isolation and characterization of antibodies targeting this recently emerged pathogen. To develop such probes, we designed constructs incorporating an N-terminal purification tag, a site-spe...

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Detalles Bibliográficos
Autores principales: Zhou, Tongqing, Teng, I-Ting, Olia, Adam S., Cerutti, Gabriele, Gorman, Jason, Nazzari, Alexandra, Shi, Wei, Tsybovsky, Yaroslav, Wang, Lingshu, Wang, Shuishu, Zhang, Baoshan, Zhang, Yi, Katsamba, Phinikoula S., Petrova, Yuliya, Banach, Bailey B., Fahad, Ahmed S., Liu, Lihong, Lopez Acevedo, Sheila N., Madan, Bharat, de Souza, Matheus Oliveira, Pan, Xiaoli, Wang, Pengfei, Wolfe, Jacy R., Yin, Michael, Ho, David D., Phung, Emily, DiPiazza, Anthony, Chang, Lauren, Abiona, Olubukula, Corbett, Kizzmekia S., DeKosky, Brandon J., Graham, Barney S., Mascola, John R., Misasi, John, Ruckwardt, Tracy, Sullivan, Nancy J., Shapiro, Lawrence, Kwong, Peter D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315997/
https://www.ncbi.nlm.nih.gov/pubmed/32596696
http://dx.doi.org/10.1101/2020.06.22.166033

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315997/
https://www.ncbi.nlm.nih.gov/pubmed/32596696
http://dx.doi.org/10.1101/2020.06.22.166033

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