Computational and structure-guided design of phosphoinositide substrate specificity into the tyrosine specific LMW-PTP enzyme

We have used a combination of computational and structure-based redesign of the low molecular weight protein tyrosine phosphatase, LMW-PTP, to create new activity towards phosphoinositide substrates for which the wild-type enzyme had little or no activity. The redesigned enzymes retain catalytic act...

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Detalles Bibliográficos
Autores principales: Egbe, Eyong, Levy, Colin W., Tabernero, Lydia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7316235/
https://www.ncbi.nlm.nih.gov/pubmed/32584877
http://dx.doi.org/10.1371/journal.pone.0235133

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7316235/
https://www.ncbi.nlm.nih.gov/pubmed/32584877
http://dx.doi.org/10.1371/journal.pone.0235133

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