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A Melanin bleaching method to prevent non-specific immunostaining of chicken feathers

Melanin in pigmented organs like the skin is known to react with 3,3′-diaminobenzidine (DAB) to give a brown colour indistinguishable from the colour that DAB imparts to target antibodies bound to specific antigens. This can lead to false positives in chicken feathers during immunoperoxidase stainin...

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Detalles Bibliográficos
Autores principales: Nazir, Shahid, Charlesworth, Richard P.G., Walkden-Brown, Stephen W., Gerber, Priscilla F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317669/
https://www.ncbi.nlm.nih.gov/pubmed/32612940
http://dx.doi.org/10.1016/j.mex.2020.100957