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A Melanin bleaching method to prevent non-specific immunostaining of chicken feathers
Melanin in pigmented organs like the skin is known to react with 3,3′-diaminobenzidine (DAB) to give a brown colour indistinguishable from the colour that DAB imparts to target antibodies bound to specific antigens. This can lead to false positives in chicken feathers during immunoperoxidase stainin...
Autores principales: | Nazir, Shahid, Charlesworth, Richard P.G., Walkden-Brown, Stephen W., Gerber, Priscilla F. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317669/ https://www.ncbi.nlm.nih.gov/pubmed/32612940 http://dx.doi.org/10.1016/j.mex.2020.100957 |
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