A Pronucleotide Probe for Live‐Cell Imaging of Protein AMPylation

Conjugation of proteins to AMP (AMPylation) is a prevalent post‐translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profili...

Descripción completa

Detalles Bibliográficos
Autores principales: Kielkowski, Pavel, Buchsbaum, Isabel Y., Becker, Tobias, Bach, Kathrin, Cappello, Silvia, Sieber, Stephan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317759/
https://www.ncbi.nlm.nih.gov/pubmed/32027064
http://dx.doi.org/10.1002/cbic.201900716
_version_ 1783550700314165248
author Kielkowski, Pavel
Buchsbaum, Isabel Y.
Becker, Tobias
Bach, Kathrin
Cappello, Silvia
Sieber, Stephan A.
author_facet Kielkowski, Pavel
Buchsbaum, Isabel Y.
Becker, Tobias
Bach, Kathrin
Cappello, Silvia
Sieber, Stephan A.
author_sort Kielkowski, Pavel
collection PubMed
description Conjugation of proteins to AMP (AMPylation) is a prevalent post‐translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain‐promoted azide–alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.
format Online
Article
Text
id pubmed-7317759
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-73177592020-06-29 A Pronucleotide Probe for Live‐Cell Imaging of Protein AMPylation Kielkowski, Pavel Buchsbaum, Isabel Y. Becker, Tobias Bach, Kathrin Cappello, Silvia Sieber, Stephan A. Chembiochem Communications Conjugation of proteins to AMP (AMPylation) is a prevalent post‐translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain‐promoted azide–alkyne click chemistry, the probe provides stable fluorescence labelling in living cells. John Wiley and Sons Inc. 2020-02-06 2020-05-04 /pmc/articles/PMC7317759/ /pubmed/32027064 http://dx.doi.org/10.1002/cbic.201900716 Text en © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Communications
Kielkowski, Pavel
Buchsbaum, Isabel Y.
Becker, Tobias
Bach, Kathrin
Cappello, Silvia
Sieber, Stephan A.
A Pronucleotide Probe for Live‐Cell Imaging of Protein AMPylation
title A Pronucleotide Probe for Live‐Cell Imaging of Protein AMPylation
title_full A Pronucleotide Probe for Live‐Cell Imaging of Protein AMPylation
title_fullStr A Pronucleotide Probe for Live‐Cell Imaging of Protein AMPylation
title_full_unstemmed A Pronucleotide Probe for Live‐Cell Imaging of Protein AMPylation
title_short A Pronucleotide Probe for Live‐Cell Imaging of Protein AMPylation
title_sort pronucleotide probe for live‐cell imaging of protein ampylation
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317759/
https://www.ncbi.nlm.nih.gov/pubmed/32027064
http://dx.doi.org/10.1002/cbic.201900716
work_keys_str_mv AT kielkowskipavel apronucleotideprobeforlivecellimagingofproteinampylation
AT buchsbaumisabely apronucleotideprobeforlivecellimagingofproteinampylation
AT beckertobias apronucleotideprobeforlivecellimagingofproteinampylation
AT bachkathrin apronucleotideprobeforlivecellimagingofproteinampylation
AT cappellosilvia apronucleotideprobeforlivecellimagingofproteinampylation
AT sieberstephana apronucleotideprobeforlivecellimagingofproteinampylation
AT kielkowskipavel pronucleotideprobeforlivecellimagingofproteinampylation
AT buchsbaumisabely pronucleotideprobeforlivecellimagingofproteinampylation
AT beckertobias pronucleotideprobeforlivecellimagingofproteinampylation
AT bachkathrin pronucleotideprobeforlivecellimagingofproteinampylation
AT cappellosilvia pronucleotideprobeforlivecellimagingofproteinampylation
AT sieberstephana pronucleotideprobeforlivecellimagingofproteinampylation

Ejemplares similares