Cargando…

Fluorescently Labelled ATP Analogues for Direct Monitoring of Ubiquitin Activation

Simple and robust assays to monitor enzymatic ATP cleavage with high efficiency in real‐time are scarce. To address this shortcoming, we developed fluorescently labelled adenosine tri‐, tetra‐ and pentaphosphate analogues of ATP. The novel ATP analogues bear — in contrast to earlier reports — only a...

Descripción completa

Detalles Bibliográficos
Autores principales: Hammler, Daniel, Stuber, Katrin, Offensperger, Fabian, Scheffner, Martin, Zumbusch, Andreas, Marx, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317923/
https://www.ncbi.nlm.nih.gov/pubmed/32154932
http://dx.doi.org/10.1002/chem.202001091