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Fluorescently Labelled ATP Analogues for Direct Monitoring of Ubiquitin Activation
Simple and robust assays to monitor enzymatic ATP cleavage with high efficiency in real‐time are scarce. To address this shortcoming, we developed fluorescently labelled adenosine tri‐, tetra‐ and pentaphosphate analogues of ATP. The novel ATP analogues bear — in contrast to earlier reports — only a...
Autores principales: | Hammler, Daniel, Stuber, Katrin, Offensperger, Fabian, Scheffner, Martin, Zumbusch, Andreas, Marx, Andreas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317923/ https://www.ncbi.nlm.nih.gov/pubmed/32154932 http://dx.doi.org/10.1002/chem.202001091 |
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