Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine

Accurate DNA quantitation is a prerequisite in many biomedical and pharmaceutical studies. Here we established a new DNA quantitation method by nuclease P1 digestion and UPLC-MS/MS analysis. DNA fragments can be efficiently hydrolyzed to single deoxyribonucleotides by nuclease P1 in a short time. Th...

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Detalles Bibliográficos
Autores principales: Ma, Yong, Chen, Buyun, Zhang, Donglu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2020
Materias:
Original article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322756/
https://www.ncbi.nlm.nih.gov/pubmed/32612871
http://dx.doi.org/10.1016/j.jpha.2020.05.011

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322756/
https://www.ncbi.nlm.nih.gov/pubmed/32612871
http://dx.doi.org/10.1016/j.jpha.2020.05.011

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