Proof-of-concept for CRISPR/Cas9 gene editing in human preadipocytes: Deletion of FKBP5 and PPARG and effects on adipocyte differentiation and metabolism

CRISPR/Cas9 has revolutionized the genome-editing field. So far, successful application in human adipose tissue has not been convincingly shown. We present a method for gene knockout using electroporation in preadipocytes from human adipose tissue that achieved at least 90% efficiency without any ne...

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Detalles Bibliográficos
Autores principales: Kamble, Prasad G., Hetty, Susanne, Vranic, Milica, Almby, Kristina, Castillejo-López, Casimiro, Abalo, Xesús M., Pereira, Maria J., Eriksson, Jan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324390/
https://www.ncbi.nlm.nih.gov/pubmed/32601291
http://dx.doi.org/10.1038/s41598-020-67293-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324390/
https://www.ncbi.nlm.nih.gov/pubmed/32601291
http://dx.doi.org/10.1038/s41598-020-67293-y

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