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In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents

The cytotoxic mechanism of the saponin QS-21 and its aglycone quillaic acid (QA) was studied on human gastric cancer cells (SNU1 and KATO III). Both compounds showed in vitro cytotoxic activity with IC(50) values: 7.1 μM (QS-21) and 13.6 μM (QA) on SNU1 cells; 7.4 μM (QS-21) and 67 μM (QA) on KATO I...

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Autores principales: Guzmán, Leda, Villalón, Katherine, Marchant, María José, Tarnok, María Elena, Cárdenas, Pilar, Aquea, Gisela, Acevedo, Waldo, Padilla, Leandro, Bernal, Giuliano, Molinari, Aurora, Corvalán, Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324585/
https://www.ncbi.nlm.nih.gov/pubmed/32601436
http://dx.doi.org/10.1038/s41598-020-67442-3
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author Guzmán, Leda
Villalón, Katherine
Marchant, María José
Tarnok, María Elena
Cárdenas, Pilar
Aquea, Gisela
Acevedo, Waldo
Padilla, Leandro
Bernal, Giuliano
Molinari, Aurora
Corvalán, Alejandro
author_facet Guzmán, Leda
Villalón, Katherine
Marchant, María José
Tarnok, María Elena
Cárdenas, Pilar
Aquea, Gisela
Acevedo, Waldo
Padilla, Leandro
Bernal, Giuliano
Molinari, Aurora
Corvalán, Alejandro
author_sort Guzmán, Leda
collection PubMed
description The cytotoxic mechanism of the saponin QS-21 and its aglycone quillaic acid (QA) was studied on human gastric cancer cells (SNU1 and KATO III). Both compounds showed in vitro cytotoxic activity with IC(50) values: 7.1 μM (QS-21) and 13.6 μM (QA) on SNU1 cells; 7.4 μM (QS-21) and 67 μM (QA) on KATO III cells. QS-21 and QA induce apoptosis on SNU1 and KATO III, as demonstrated by TUNEL, Annexin-V and Caspase Assays. Additionally, we performed in silico docking studies simulating the binding of both triterpenic compounds to key proteins involved in apoptotic pathways. The binding energies (∆G(bin)) thus calculated, suggest that the pro-apoptotic protein Bid might be a plausible target involved in the apoptotic effect of both triterpenic compounds. Although QA shows some antiproliferative effects on SNU1 cells cultured in vitro, our results suggest that QS-21 is a more powerful antitumor agent, which merits further investigation regarding their properties as potential therapeutic agents for gastric cancer.
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spelling pubmed-73245852020-07-01 In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents Guzmán, Leda Villalón, Katherine Marchant, María José Tarnok, María Elena Cárdenas, Pilar Aquea, Gisela Acevedo, Waldo Padilla, Leandro Bernal, Giuliano Molinari, Aurora Corvalán, Alejandro Sci Rep Article The cytotoxic mechanism of the saponin QS-21 and its aglycone quillaic acid (QA) was studied on human gastric cancer cells (SNU1 and KATO III). Both compounds showed in vitro cytotoxic activity with IC(50) values: 7.1 μM (QS-21) and 13.6 μM (QA) on SNU1 cells; 7.4 μM (QS-21) and 67 μM (QA) on KATO III cells. QS-21 and QA induce apoptosis on SNU1 and KATO III, as demonstrated by TUNEL, Annexin-V and Caspase Assays. Additionally, we performed in silico docking studies simulating the binding of both triterpenic compounds to key proteins involved in apoptotic pathways. The binding energies (∆G(bin)) thus calculated, suggest that the pro-apoptotic protein Bid might be a plausible target involved in the apoptotic effect of both triterpenic compounds. Although QA shows some antiproliferative effects on SNU1 cells cultured in vitro, our results suggest that QS-21 is a more powerful antitumor agent, which merits further investigation regarding their properties as potential therapeutic agents for gastric cancer. Nature Publishing Group UK 2020-06-29 /pmc/articles/PMC7324585/ /pubmed/32601436 http://dx.doi.org/10.1038/s41598-020-67442-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Guzmán, Leda
Villalón, Katherine
Marchant, María José
Tarnok, María Elena
Cárdenas, Pilar
Aquea, Gisela
Acevedo, Waldo
Padilla, Leandro
Bernal, Giuliano
Molinari, Aurora
Corvalán, Alejandro
In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents
title In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents
title_full In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents
title_fullStr In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents
title_full_unstemmed In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents
title_short In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents
title_sort in vitro evaluation and molecular docking of qs-21 and quillaic acid from quillaja saponaria molina as gastric cancer agents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324585/
https://www.ncbi.nlm.nih.gov/pubmed/32601436
http://dx.doi.org/10.1038/s41598-020-67442-3
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