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SnoRNA signatures in cartilage ageing and osteoarthritis
Osteoarthritis presents as a change in the chondrocyte phenotype and an imbalance between anabolic and catabolic processes. Age affects its onset and progression. Small nucleolar RNAs (SnoRNAs) direct chemical modification of RNA substrates to fine-tune spliceosomal and rRNA function, accommodating...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326970/ https://www.ncbi.nlm.nih.gov/pubmed/32606371 http://dx.doi.org/10.1038/s41598-020-67446-z |
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author | Peffers, Mandy J. Chabronova, Alzbeta Balaskas, Panagiotis Fang, Yongxiang Dyer, Philip Cremers, Andy Emans, Pieter J. Feczko, Peter Z. Caron, Marjolein M. Welting, Tim J. M. |
author_facet | Peffers, Mandy J. Chabronova, Alzbeta Balaskas, Panagiotis Fang, Yongxiang Dyer, Philip Cremers, Andy Emans, Pieter J. Feczko, Peter Z. Caron, Marjolein M. Welting, Tim J. M. |
author_sort | Peffers, Mandy J. |
collection | PubMed |
description | Osteoarthritis presents as a change in the chondrocyte phenotype and an imbalance between anabolic and catabolic processes. Age affects its onset and progression. Small nucleolar RNAs (SnoRNAs) direct chemical modification of RNA substrates to fine-tune spliceosomal and rRNA function, accommodating changing requirements for splicing and protein synthesis during health and disease. Articular cartilage from young, old and OA knees was used in a microarray study to identify alterations in snoRNA expression. Changes in snoRNAs in osteoarthritis-like conditions were studied in chondrocytes using interleukin-1 and osteoarthritic synovial fluid. SNORD26 and SNORD96A knockdown and overexpression were undertaken using antisense oligonucleotides and overexpression plasmids. We identified panels of snoRNAs differentially expressed due to ageing (including SNORD96A, SNORD44) and osteoarthritis (including SNORD26 and SNORD116). In vitro experiments using osteoarthritis-like conditions affected snoRNA expression. Knockdown or overexpression of SNORD26 or SNORD96A resulted in changes in chondrogenic, hypertrophic, rRNA and osteoarthritis related gene expression. We demonstrate that snoRNA expression changes in cartilage ageing, and osteoarthritis and in osteoarthritis-like conditions, and when the expression of these snoRNAs is altered this affects chondrogenic and hypertrophic gene expression. Thus, we propose an additional dimension in the molecular mechanisms underlying cartilage ageing and osteoarthritis through the dysregulation of snoRNAs. |
format | Online Article Text |
id | pubmed-7326970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73269702020-07-01 SnoRNA signatures in cartilage ageing and osteoarthritis Peffers, Mandy J. Chabronova, Alzbeta Balaskas, Panagiotis Fang, Yongxiang Dyer, Philip Cremers, Andy Emans, Pieter J. Feczko, Peter Z. Caron, Marjolein M. Welting, Tim J. M. Sci Rep Article Osteoarthritis presents as a change in the chondrocyte phenotype and an imbalance between anabolic and catabolic processes. Age affects its onset and progression. Small nucleolar RNAs (SnoRNAs) direct chemical modification of RNA substrates to fine-tune spliceosomal and rRNA function, accommodating changing requirements for splicing and protein synthesis during health and disease. Articular cartilage from young, old and OA knees was used in a microarray study to identify alterations in snoRNA expression. Changes in snoRNAs in osteoarthritis-like conditions were studied in chondrocytes using interleukin-1 and osteoarthritic synovial fluid. SNORD26 and SNORD96A knockdown and overexpression were undertaken using antisense oligonucleotides and overexpression plasmids. We identified panels of snoRNAs differentially expressed due to ageing (including SNORD96A, SNORD44) and osteoarthritis (including SNORD26 and SNORD116). In vitro experiments using osteoarthritis-like conditions affected snoRNA expression. Knockdown or overexpression of SNORD26 or SNORD96A resulted in changes in chondrogenic, hypertrophic, rRNA and osteoarthritis related gene expression. We demonstrate that snoRNA expression changes in cartilage ageing, and osteoarthritis and in osteoarthritis-like conditions, and when the expression of these snoRNAs is altered this affects chondrogenic and hypertrophic gene expression. Thus, we propose an additional dimension in the molecular mechanisms underlying cartilage ageing and osteoarthritis through the dysregulation of snoRNAs. Nature Publishing Group UK 2020-06-30 /pmc/articles/PMC7326970/ /pubmed/32606371 http://dx.doi.org/10.1038/s41598-020-67446-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Peffers, Mandy J. Chabronova, Alzbeta Balaskas, Panagiotis Fang, Yongxiang Dyer, Philip Cremers, Andy Emans, Pieter J. Feczko, Peter Z. Caron, Marjolein M. Welting, Tim J. M. SnoRNA signatures in cartilage ageing and osteoarthritis |
title | SnoRNA signatures in cartilage ageing and osteoarthritis |
title_full | SnoRNA signatures in cartilage ageing and osteoarthritis |
title_fullStr | SnoRNA signatures in cartilage ageing and osteoarthritis |
title_full_unstemmed | SnoRNA signatures in cartilage ageing and osteoarthritis |
title_short | SnoRNA signatures in cartilage ageing and osteoarthritis |
title_sort | snorna signatures in cartilage ageing and osteoarthritis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326970/ https://www.ncbi.nlm.nih.gov/pubmed/32606371 http://dx.doi.org/10.1038/s41598-020-67446-z |
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