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Analysis of the influence of flame sterilization included in sampling operations on shake-flask cultures of microorganisms

Shake-flask cultures of microorganisms involve flame sterilization during sampling, which produces combustion gas with high CO(2) concentrations. The gaseous destination has not been deeply analyzed. Our aim was to investigate the effect of flame sterilization on the headspace of the flask and on th...

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Detalles Bibliográficos
Autores principales: Takahashi, Masato, Honzawa, Takafumi, Tominaga, Ryuichi, Aoyagi, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326993/
https://www.ncbi.nlm.nih.gov/pubmed/32606322
http://dx.doi.org/10.1038/s41598-020-66810-3