Cargando…
SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485
Aging is a major risk factor for hypertension and atherosclerosis, and vascular smooth muscle cell (VSMC) senescence can promote aging‐related vascular diseases. Sirtuin‐1 (SIRT1) and AMP‐activated protein kinase (AMPK) were previously reported to modulate vascular senescence; however, its effects h...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327920/ https://www.ncbi.nlm.nih.gov/pubmed/32421926 http://dx.doi.org/10.1002/2211-5463.12895 |
_version_ | 1783552651334516736 |
---|---|
author | Sung, Jin Young Kim, Seul Gi Cho, Du Hyong Kim, Jae‐Ryong Choi, Hyoung Chul |
author_facet | Sung, Jin Young Kim, Seul Gi Cho, Du Hyong Kim, Jae‐Ryong Choi, Hyoung Chul |
author_sort | Sung, Jin Young |
collection | PubMed |
description | Aging is a major risk factor for hypertension and atherosclerosis, and vascular smooth muscle cell (VSMC) senescence can promote aging‐related vascular diseases. Sirtuin‐1 (SIRT1) and AMP‐activated protein kinase (AMPK) were previously reported to modulate vascular senescence; however, its effects have not been well characterized. To determine the nature of the interaction between SIRT1 and AMPK in VSMC senescence, we investigated the effects of SRT1720 on its downstream targets of SIRT1 and the phosphorylation of AMPKα at Ser485. During Adriamycin‐induced VSMC senescence, SRT1720 increased the activity of SIRT1 and AMPKα phosphorylation at Ser485 via the cAMP–protein kinase A (PKA) pathway. Telomere length and telomerase reverse transcriptase expression were increased by SIRT1 activation with SRT1720. Taken together, these data show that activation of the SIRT1/cAMP–PKA/p‐AMPKα (Ser485) pathway may be an effective antisenescence mechanism for VSMCs. |
format | Online Article Text |
id | pubmed-7327920 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73279202020-07-02 SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 Sung, Jin Young Kim, Seul Gi Cho, Du Hyong Kim, Jae‐Ryong Choi, Hyoung Chul FEBS Open Bio Research Articles Aging is a major risk factor for hypertension and atherosclerosis, and vascular smooth muscle cell (VSMC) senescence can promote aging‐related vascular diseases. Sirtuin‐1 (SIRT1) and AMP‐activated protein kinase (AMPK) were previously reported to modulate vascular senescence; however, its effects have not been well characterized. To determine the nature of the interaction between SIRT1 and AMPK in VSMC senescence, we investigated the effects of SRT1720 on its downstream targets of SIRT1 and the phosphorylation of AMPKα at Ser485. During Adriamycin‐induced VSMC senescence, SRT1720 increased the activity of SIRT1 and AMPKα phosphorylation at Ser485 via the cAMP–protein kinase A (PKA) pathway. Telomere length and telomerase reverse transcriptase expression were increased by SIRT1 activation with SRT1720. Taken together, these data show that activation of the SIRT1/cAMP–PKA/p‐AMPKα (Ser485) pathway may be an effective antisenescence mechanism for VSMCs. John Wiley and Sons Inc. 2020-06-05 /pmc/articles/PMC7327920/ /pubmed/32421926 http://dx.doi.org/10.1002/2211-5463.12895 Text en © 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Sung, Jin Young Kim, Seul Gi Cho, Du Hyong Kim, Jae‐Ryong Choi, Hyoung Chul SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 |
title | SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 |
title_full | SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 |
title_fullStr | SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 |
title_full_unstemmed | SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 |
title_short | SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 |
title_sort | srt1720‐induced activation of sirt1 alleviates vascular smooth muscle cell senescence through pka‐dependent phosphorylation of ampkα at ser485 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327920/ https://www.ncbi.nlm.nih.gov/pubmed/32421926 http://dx.doi.org/10.1002/2211-5463.12895 |
work_keys_str_mv | AT sungjinyoung srt1720inducedactivationofsirt1alleviatesvascularsmoothmusclecellsenescencethroughpkadependentphosphorylationofampkaatser485 AT kimseulgi srt1720inducedactivationofsirt1alleviatesvascularsmoothmusclecellsenescencethroughpkadependentphosphorylationofampkaatser485 AT choduhyong srt1720inducedactivationofsirt1alleviatesvascularsmoothmusclecellsenescencethroughpkadependentphosphorylationofampkaatser485 AT kimjaeryong srt1720inducedactivationofsirt1alleviatesvascularsmoothmusclecellsenescencethroughpkadependentphosphorylationofampkaatser485 AT choihyoungchul srt1720inducedactivationofsirt1alleviatesvascularsmoothmusclecellsenescencethroughpkadependentphosphorylationofampkaatser485 |