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Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron

Graded transcription factors are pivotal regulators of embryonic patterning, but whether their role changes over time is unclear. A light-regulated protein degradation system was used to assay temporal dependence of the transcription factor Dorsal in dorsal–ventral axis patterning of Drosophila embr...

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Autores principales: Irizarry, Jihyun, McGehee, James, Kim, Goheun, Stein, David, Stathopoulos, Angelike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7328519/
https://www.ncbi.nlm.nih.gov/pubmed/32467225
http://dx.doi.org/10.1101/gad.338194.120
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author Irizarry, Jihyun
McGehee, James
Kim, Goheun
Stein, David
Stathopoulos, Angelike
author_facet Irizarry, Jihyun
McGehee, James
Kim, Goheun
Stein, David
Stathopoulos, Angelike
author_sort Irizarry, Jihyun
collection PubMed
description Graded transcription factors are pivotal regulators of embryonic patterning, but whether their role changes over time is unclear. A light-regulated protein degradation system was used to assay temporal dependence of the transcription factor Dorsal in dorsal–ventral axis patterning of Drosophila embryos. Surprisingly, the high-threshold target gene snail only requires Dorsal input early but not late when Dorsal levels peak. Instead, late snail expression can be supported by action of the Twist transcription factor, specifically, through one enhancer, sna.distal. This study demonstrates that continuous input is not required for some Dorsal targets and downstream responses, such as twist, function as molecular ratchets.
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spelling pubmed-73285192021-01-01 Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron Irizarry, Jihyun McGehee, James Kim, Goheun Stein, David Stathopoulos, Angelike Genes Dev Research Paper Graded transcription factors are pivotal regulators of embryonic patterning, but whether their role changes over time is unclear. A light-regulated protein degradation system was used to assay temporal dependence of the transcription factor Dorsal in dorsal–ventral axis patterning of Drosophila embryos. Surprisingly, the high-threshold target gene snail only requires Dorsal input early but not late when Dorsal levels peak. Instead, late snail expression can be supported by action of the Twist transcription factor, specifically, through one enhancer, sna.distal. This study demonstrates that continuous input is not required for some Dorsal targets and downstream responses, such as twist, function as molecular ratchets. Cold Spring Harbor Laboratory Press 2020-07-01 /pmc/articles/PMC7328519/ /pubmed/32467225 http://dx.doi.org/10.1101/gad.338194.120 Text en © 2020 Irizarry et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Paper
Irizarry, Jihyun
McGehee, James
Kim, Goheun
Stein, David
Stathopoulos, Angelike
Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron
title Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron
title_full Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron
title_fullStr Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron
title_full_unstemmed Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron
title_short Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron
title_sort twist-dependent ratchet functioning downstream from dorsal revealed using a light-inducible degron
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7328519/
https://www.ncbi.nlm.nih.gov/pubmed/32467225
http://dx.doi.org/10.1101/gad.338194.120
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