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Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron
Graded transcription factors are pivotal regulators of embryonic patterning, but whether their role changes over time is unclear. A light-regulated protein degradation system was used to assay temporal dependence of the transcription factor Dorsal in dorsal–ventral axis patterning of Drosophila embr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7328519/ https://www.ncbi.nlm.nih.gov/pubmed/32467225 http://dx.doi.org/10.1101/gad.338194.120 |
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author | Irizarry, Jihyun McGehee, James Kim, Goheun Stein, David Stathopoulos, Angelike |
author_facet | Irizarry, Jihyun McGehee, James Kim, Goheun Stein, David Stathopoulos, Angelike |
author_sort | Irizarry, Jihyun |
collection | PubMed |
description | Graded transcription factors are pivotal regulators of embryonic patterning, but whether their role changes over time is unclear. A light-regulated protein degradation system was used to assay temporal dependence of the transcription factor Dorsal in dorsal–ventral axis patterning of Drosophila embryos. Surprisingly, the high-threshold target gene snail only requires Dorsal input early but not late when Dorsal levels peak. Instead, late snail expression can be supported by action of the Twist transcription factor, specifically, through one enhancer, sna.distal. This study demonstrates that continuous input is not required for some Dorsal targets and downstream responses, such as twist, function as molecular ratchets. |
format | Online Article Text |
id | pubmed-7328519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-73285192021-01-01 Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron Irizarry, Jihyun McGehee, James Kim, Goheun Stein, David Stathopoulos, Angelike Genes Dev Research Paper Graded transcription factors are pivotal regulators of embryonic patterning, but whether their role changes over time is unclear. A light-regulated protein degradation system was used to assay temporal dependence of the transcription factor Dorsal in dorsal–ventral axis patterning of Drosophila embryos. Surprisingly, the high-threshold target gene snail only requires Dorsal input early but not late when Dorsal levels peak. Instead, late snail expression can be supported by action of the Twist transcription factor, specifically, through one enhancer, sna.distal. This study demonstrates that continuous input is not required for some Dorsal targets and downstream responses, such as twist, function as molecular ratchets. Cold Spring Harbor Laboratory Press 2020-07-01 /pmc/articles/PMC7328519/ /pubmed/32467225 http://dx.doi.org/10.1101/gad.338194.120 Text en © 2020 Irizarry et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Paper Irizarry, Jihyun McGehee, James Kim, Goheun Stein, David Stathopoulos, Angelike Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron |
title | Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron |
title_full | Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron |
title_fullStr | Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron |
title_full_unstemmed | Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron |
title_short | Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron |
title_sort | twist-dependent ratchet functioning downstream from dorsal revealed using a light-inducible degron |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7328519/ https://www.ncbi.nlm.nih.gov/pubmed/32467225 http://dx.doi.org/10.1101/gad.338194.120 |
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