Cargando…

A programmable CRISPR/Cas9-based phage defense system for Escherichia coli BL21(DE3)

Escherichia coli BL21 is arguably the most popular host for industrial production of proteins, and industrial fermentations are often plagued by phage infections. The CRISPR/Cas system is guided by a gRNA to cleave a specific DNA cassette, which can be developed into a highly efficient programable p...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liu, Li, Zhao, Dongdong, Ye, Lijun, Zhan, Tao, Xiong, Bin, Hu, Muzi, Bi, Changhao, Zhang, Xueli
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7332536/ https://www.ncbi.nlm.nih.gov/pubmed/32620105 http://dx.doi.org/10.1186/s12934-020-01393-2

Ejemplares similares

Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9
por: Zhao, Dongdong, et al.
Publicado: (2016)

A CRISPR-based chromosomal-separation technique for Escherichia coli
por: Su, Junchang, et al.
Publicado: (2022)

Artificial Diploid Escherichia coli by a CRISPR Chromosome‐Doubling Technique
por: Wang, Pengju, et al.
Publicado: (2023)

Metabolic engineering of Escherichia coli BL21 strain using simplified CRISPR-Cas9 and asymmetric homology arms recombineering
por: Shukal, Sudha, et al.
Publicado: (2022)

Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique
por: Xiong, Bin, et al.
Publicado: (2018)

Construction of Escherichia coli cell factories for crocin biosynthesis
por: Wang, Wen, et al.
Publicado: (2019)

CRISPR-Cas adaptation in Escherichia coli
por: Mitić, Damjan, et al.
Publicado: (2023)

Construction of a novel anaerobic pathway in Escherichia coli for propionate production
por: Li, Jing, et al.
Publicado: (2017)

CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency
por: Zhao, Dongdong, et al.
Publicado: (2017)

CRISPR/Cas Technologies and Their Applications in Escherichia coli
por: Dong, Huina, et al.
Publicado: (2021)

Combinatory optimization of chromosomal integrated mevalonate pathway for β-carotene production in Escherichia coli
por: Ye, Lijun, et al.
Publicado: (2016)

Combinatorial modulation of initial codons for improved zeaxanthin synthetic pathway efficiency in Escherichia coli
por: Wu, Zaiqiang, et al.
Publicado: (2019)

Complete Genome Sequence of Escherichia coli Strain BL21
por: Jeong, Haeyoung, et al.
Publicado: (2015)

Complete Genome Sequence of Escherichia coli BL21-AI
por: Bhawsinghka, Niketa, et al.
Publicado: (2020)

CRISPR-Cas systems in the marine actinomycete Salinispora: linkages with phage defense, microdiversity and biogeography
por: Wietz, Matthias, et al.
Publicado: (2014)

H-NS Mutation-Mediated CRISPR-Cas Activation Inhibits Phage Release and Toxin Production of Escherichia coli Stx2 Phage Lysogen
por: Fu, Qiang, et al.
Publicado: (2017)

Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3
por: Yoshimi, Kazuto, et al.
Publicado: (2022)

CRISPR-Cas Functional Module Exchange in Escherichia coli
por: Almendros, Cristóbal, et al.
Publicado: (2014)

Mobile CRISPR-Cas9 based anti-phage system in E. coli
por: Cao, Zhou, et al.
Publicado: (2022)

The metabolic potential of Escherichia coli BL21 in defined and rich medium
por: Li, Zhaopeng, et al.
Publicado: (2014)

Genomic and transcriptomic landscape of Escherichia coli BL21(DE3)
por: Kim, Sinyeon, et al.
Publicado: (2017)

Manipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency
por: Xie, Qianwen, et al.
Publicado: (2020)

Impact of the Expression System on Recombinant Protein Production in Escherichia coli BL21
por: Lozano Terol, Gema, et al.
Publicado: (2021)

Anti-CRISPR Phages Cooperate to Overcome CRISPR-Cas Immunity
por: Landsberger, Mariann, et al.
Publicado: (2018)

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli
por: Sapranauskas, Rimantas, et al.
Publicado: (2011)

Transcriptional profiling of thymidine-producing strain recombineered from Escherichia coli BL21
por: Kim, Jin-Sook, et al.
Publicado: (2015)

A systematic optimization of styrene biosynthesis in Escherichia coli BL21(DE3)
por: Liu, Changqing, et al.
Publicado: (2018)

Enhancing the biosynthesis of riboflavin in the recombinant Escherichia coli BL21 strain by metabolic engineering
por: Fu, Bing, et al.
Publicado: (2023)

Identification of a novel family B DNA polymerase from Enterococcus phage IME199 and its overproduction in Escherichia coli BL21(DE3)
por: Han, Pengjun, et al.
Publicado: (2023)

Phage Genetic Engineering Using CRISPR–Cas Systems
por: Hatoum-Aslan, Asma
Publicado: (2018)

Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO(2)
por: Wu, Zaiqiang, et al.
Publicado: (2019)

Physiological Response of Escherichia coli W3110 and BL21 to the Aerobic Expression of Vitreoscilla Hemoglobin
por: Lara, Alvaro R., et al.
Publicado: (2020)

Programmable Biosensors Based on RNA-Guided CRISPR/Cas Endonuclease
por: Liu, Xiaolong, et al.
Publicado: (2022)

CRISPR/Cas9-Mediated Phage Resistance Is Not Impeded by the DNA Modifications of Phage T4
por: Yaung, Stephanie J., et al.
Publicado: (2014)

Phage engineering and phage‐assisted CRISPR‐Cas delivery to combat multidrug‐resistant pathogens
por: Khambhati, Khushal, et al.
Publicado: (2022)

Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS
por: Majsec, Kristina, et al.
Publicado: (2016)

Characterization, complete genome sequencing, and CRISPR/Cas9 system-based decontamination of a novel Escherichia coli phage TR1 from fermentation substrates
por: Dong, Yuqi, et al.
Publicado: (2023)

Exploring the ecological function of CRISPR-Cas virus defense
por: Lundgren, Magnus
Publicado: (2016)

Mucin induces CRISPR-Cas defense in an opportunistic pathogen
por: de Freitas Almeida, Gabriel Magno, et al.
Publicado: (2022)

Optimization of T4 phage engineering via CRISPR/Cas9
por: Duong, Michelle M., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_5ncdm9c61i933he2aeh21eopmj