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Reconstituted cell-free protein synthesis using in vitro transcribed tRNAs

Entire reconstitution of tRNAs for active protein production in a cell-free system brings flexibility into the genetic code engineering. It can also contribute to the field of cell-free synthetic biology, which aims to construct self-replicable artificial cells. Herein, we developed a system equippe...

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Detalles Bibliográficos
Autores principales: Hibi, Keita, Amikura, Kazuaki, Sugiura, Naoki, Masuda, Keiko, Ohno, Satoshi, Yokogawa, Takashi, Ueda, Takuya, Shimizu, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334211/
https://www.ncbi.nlm.nih.gov/pubmed/32620935
http://dx.doi.org/10.1038/s42003-020-1074-2