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Two particle-picking procedures for filamentous proteins: SPHIRE-crYOLO filament mode and SPHIRE-STRIPER

Structure determination of filamentous molecular complexes involves the selection of filaments from cryo-EM micrographs. The automatic selection of helical specimens is particularly difficult, and thus many challenging samples with issues such as contamination or aggregation are still manually picke...

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Detalles Bibliográficos
Autores principales: Wagner, Thorsten, Lusnig, Luca, Pospich, Sabrina, Stabrin, Markus, Schönfeld, Fabian, Raunser, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336381/
https://www.ncbi.nlm.nih.gov/pubmed/32627734
http://dx.doi.org/10.1107/S2059798320007342