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Two particle-picking procedures for filamentous proteins: SPHIRE-crYOLO filament mode and SPHIRE-STRIPER
Structure determination of filamentous molecular complexes involves the selection of filaments from cryo-EM micrographs. The automatic selection of helical specimens is particularly difficult, and thus many challenging samples with issues such as contamination or aggregation are still manually picke...
Autores principales: | Wagner, Thorsten, Lusnig, Luca, Pospich, Sabrina, Stabrin, Markus, Schönfeld, Fabian, Raunser, Stefan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336381/ https://www.ncbi.nlm.nih.gov/pubmed/32627734 http://dx.doi.org/10.1107/S2059798320007342 |
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