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T Lymphocytes in Patients With Nijmegen Breakage Syndrome Demonstrate Features of Exhaustion and Senescence in Flow Cytometric Evaluation of Maturation Pathway

Patients with Nijmegen Breakage Syndrome (NBS) suffer from recurrent infections due to humoral and cellular immune deficiency. Despite low number of T lymphocytes and their maturation defect, the clinical manifestations of cell-mediated deficiency are not as severe as in case of patients with other...

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Autores principales: Piatosa, Barbara, Wolska-Kuśnierz, Beata, Tkaczyk, Katarzyna, Heropolitanska-Pliszka, Edyta, Grycuk, Urszula, Wakulinska, Anna, Gregorek, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338427/
https://www.ncbi.nlm.nih.gov/pubmed/32695108
http://dx.doi.org/10.3389/fimmu.2020.01319
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author Piatosa, Barbara
Wolska-Kuśnierz, Beata
Tkaczyk, Katarzyna
Heropolitanska-Pliszka, Edyta
Grycuk, Urszula
Wakulinska, Anna
Gregorek, Hanna
author_facet Piatosa, Barbara
Wolska-Kuśnierz, Beata
Tkaczyk, Katarzyna
Heropolitanska-Pliszka, Edyta
Grycuk, Urszula
Wakulinska, Anna
Gregorek, Hanna
author_sort Piatosa, Barbara
collection PubMed
description Patients with Nijmegen Breakage Syndrome (NBS) suffer from recurrent infections due to humoral and cellular immune deficiency. Despite low number of T lymphocytes and their maturation defect, the clinical manifestations of cell-mediated deficiency are not as severe as in case of patients with other types of combined immune deficiencies and similar T cell lymphopenia. In this study, multicolor flow cytometry was used for evaluation of peripheral T lymphocyte maturation according to the currently known differentiation pathway, in 46 patients with genetically confirmed NBS and 46 sex and age-matched controls. Evaluation of differential expression of CD27, CD31, CD45RA, CD95, and CD197 revealed existence of cell subsets so far not described in NBS patients. Although recent thymic emigrants and naïve T lymphocyte cell populations were significantly lower, the generation of antigen-primed T cells was similar or even greater in NBS patients than in healthy controls. Moreover, the senescent and exhausted T cell populations defined by expression of CD57, KLRG1, and PD1 were more numerous than in healthy people. Although this hypothesis needs further investigations, such properties might be related to an increased susceptibility to malignancy and milder clinical course than expected in view of T cell lymphopenia in patients with NBS.
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spelling pubmed-73384272020-07-20 T Lymphocytes in Patients With Nijmegen Breakage Syndrome Demonstrate Features of Exhaustion and Senescence in Flow Cytometric Evaluation of Maturation Pathway Piatosa, Barbara Wolska-Kuśnierz, Beata Tkaczyk, Katarzyna Heropolitanska-Pliszka, Edyta Grycuk, Urszula Wakulinska, Anna Gregorek, Hanna Front Immunol Immunology Patients with Nijmegen Breakage Syndrome (NBS) suffer from recurrent infections due to humoral and cellular immune deficiency. Despite low number of T lymphocytes and their maturation defect, the clinical manifestations of cell-mediated deficiency are not as severe as in case of patients with other types of combined immune deficiencies and similar T cell lymphopenia. In this study, multicolor flow cytometry was used for evaluation of peripheral T lymphocyte maturation according to the currently known differentiation pathway, in 46 patients with genetically confirmed NBS and 46 sex and age-matched controls. Evaluation of differential expression of CD27, CD31, CD45RA, CD95, and CD197 revealed existence of cell subsets so far not described in NBS patients. Although recent thymic emigrants and naïve T lymphocyte cell populations were significantly lower, the generation of antigen-primed T cells was similar or even greater in NBS patients than in healthy controls. Moreover, the senescent and exhausted T cell populations defined by expression of CD57, KLRG1, and PD1 were more numerous than in healthy people. Although this hypothesis needs further investigations, such properties might be related to an increased susceptibility to malignancy and milder clinical course than expected in view of T cell lymphopenia in patients with NBS. Frontiers Media S.A. 2020-06-30 /pmc/articles/PMC7338427/ /pubmed/32695108 http://dx.doi.org/10.3389/fimmu.2020.01319 Text en Copyright © 2020 Piatosa, Wolska-Kuśnierz, Tkaczyk, Heropolitanska-Pliszka, Grycuk, Wakulinska and Gregorek. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Piatosa, Barbara
Wolska-Kuśnierz, Beata
Tkaczyk, Katarzyna
Heropolitanska-Pliszka, Edyta
Grycuk, Urszula
Wakulinska, Anna
Gregorek, Hanna
T Lymphocytes in Patients With Nijmegen Breakage Syndrome Demonstrate Features of Exhaustion and Senescence in Flow Cytometric Evaluation of Maturation Pathway
title T Lymphocytes in Patients With Nijmegen Breakage Syndrome Demonstrate Features of Exhaustion and Senescence in Flow Cytometric Evaluation of Maturation Pathway
title_full T Lymphocytes in Patients With Nijmegen Breakage Syndrome Demonstrate Features of Exhaustion and Senescence in Flow Cytometric Evaluation of Maturation Pathway
title_fullStr T Lymphocytes in Patients With Nijmegen Breakage Syndrome Demonstrate Features of Exhaustion and Senescence in Flow Cytometric Evaluation of Maturation Pathway
title_full_unstemmed T Lymphocytes in Patients With Nijmegen Breakage Syndrome Demonstrate Features of Exhaustion and Senescence in Flow Cytometric Evaluation of Maturation Pathway
title_short T Lymphocytes in Patients With Nijmegen Breakage Syndrome Demonstrate Features of Exhaustion and Senescence in Flow Cytometric Evaluation of Maturation Pathway
title_sort t lymphocytes in patients with nijmegen breakage syndrome demonstrate features of exhaustion and senescence in flow cytometric evaluation of maturation pathway
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338427/
https://www.ncbi.nlm.nih.gov/pubmed/32695108
http://dx.doi.org/10.3389/fimmu.2020.01319
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