Effects of Fermented Soybean Meal Supplementation on the Growth Performance and Cecal Microbiota Community of Broiler Chickens

SIMPLE SUMMARY: Microbial fermentation is considered an economically viable processing technique to reduce the content of anti-nutritional factors and improve the nutritional quality of soybean meal (SBM). Fermented soybean meal (FSBM) exerts beneficial effects on the growth performance, carcass traits, and meat quality of broilers. However, there is very little information on the effects of FSBM on cecal microbial composition and diversity in broilers. Hence, this trial is conducted to investigate the effects of the partial replacement of SBM with FSBM in the diet on growth performance and cecal microflora of broilers. Replacing 25% of the SBM in the diet of broilers with FSBM promoted average daily gain and feed conversion ratio during the growth and whole phases. Both dietary treatment (25% or 50% of the SBM in the basal diet was replaced with FSBM) influenced the serum immunity, diversity and composition of cecal microbiota in broilers. FSBM supplementation in the diet shifted the cecal microbial community of broilers towards a healthier balance by increasing the abundance of beneficial bacteria and reducing the abundance of potentially harmful bacteria. ABSTRACT: This study investigated the growth performance, serum immunity, and cecal bacterial microbiota of broilers fed a diet in which soybean meal (SBM) was partially replaced with fermented soybean meal (FSBM) for 36 days. A total of 180 one-day-old male Cobb 500 broilers were randomly divided into three dietary groups (six replicates per group): corn-SBM diet (CC); 25% SBM replaced by FSBM (SC); 50% SBM replaced by FSBM (TC). The average daily gain (ADG) and feed conversion rates (FCR) were higher in SC than CC and TC groups (p < 0.05) during the growth (d 22–36) and whole (d 1–36) phases. No significant difference was observed in ADG and average daily feed intake (ADFI) between CC and TC groups during any phases. Dietary treatments increased serum IgA, IgG, and IgM, Chao 1, observed species, and the abundance of the phylum Fimicutes but decreased the proportion of Proteobacteria (p < 0.05). Dietary treatments increased the abundance of the genera Lachnospiraceae, Lachnoclostridium, Gastranaerophilales, and Lactobacillus but decreased the abundance of Escherichia-Shigella and Clostridiales (p < 0.05). Spearman’s correlations showed that the abundance of Gastranaerophilales was positively correlated with ADG and serum immunity, and the abundance of Lactobacillus was strongly positively with IgM. Thus, replacing 25% of SBM with FSBM improves the growth performance and serum immunity of broilers, possibly due to altered cecal microbial composition.